Recombinant Arabidopsis thaliana Solanesyl diphosphate synthase 3, chloroplastic/mitochondrial (SPS3)

Code CSB-YP705414DOA
MSDS
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Source Yeast
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Code CSB-EP705414DOA
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Source E.coli
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Code CSB-EP705414DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP705414DOA
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Source Baculovirus
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Code CSB-MP705414DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
SPS3
Uniprot No.
Alternative Names
SPS3; GPS1; At2g34630; T31E10.3; Solanesyl diphosphate synthase 3; chloroplastic/mitochondrial; EC 2.5.1.85; All-trans-nonaprenyl-diphosphate synthase 3; geranylgeranyl-diphosphate specific); Geranyl diphosphate synthase 1; Trans-type polyprenyl pyrophosphate synthase; AtPPPS
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
33-422
Target Protein Sequence
AIIPDQGH SCSDSPHKGY VCRTTYSLKS PVFGGFSHQL YHQSSSLVEE ELDPFSLVAD ELSLLSNKLR EMVLAEVPKL ASAAEYFFKR GVQGKQFRST ILLLMATALN VRVPEALIGE STDIVTSELR VRQRGIAEIT EMIHVASLLH DDVLDDADTR RGVGSLNVVM GNKMSVLAGD FLLSRACGAL AALKNTEVVA LLATAVEHLV TGETMEITSS TEQRYSMDYY MQKTYYKTAS LISNSCKAVA VLTGQTAEVA VLAFEYGRNL GLAFQLIDDI LDFTGTSASL GKGSLSDIRH GVITAPILFA MEEFPQLREV VDQVEKDPRN VDIALEYLGK SKGIQRAREL AMEHANLAAA AIGSLPETDN EDVKRSRRAL IDLTHRVITR NK
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
May be involved in the supply of solanesyl diphosphate for ubiquinone-9 (UQ-9) biosynthesis in mitochondria. Synthesizes C25 to C45 medium / long-chain products depending on the type of substrate available. Can use geranyl diphosphate, farnesyl diphosphate or geranylgeranyl diphosphate as substrates, but not dimethylallyl diphosphate.
Gene References into Functions
  1. Phylogenetic analyses revealed that At2g34630 is the only Arabidopsis trans-long-chain prenyl diphosphate synthase that clusters with the Coq1 orthologs involved in the biosynthesis of ubiquinone in other eukaryotes. PMID: 21950843
  2. A novel prenyltransferases from Arabidopsis thaliana is identified as a trans-type polyprenyl pyrophosphate synthase which is involved in the synthesis of multiple medium/long-chain products. [AtPPPS] PMID: 21220764
Subcellular Location
Plastid, chloroplast. Mitochondrion.
Protein Families
FPP/GGPP synthase family
Tissue Specificity
Ubiquitous. Highest expression in seeds and shoot apical meristem.
Database Links

KEGG: ath:AT2G34630

STRING: 3702.AT2G34630.2

UniGene: At.25503

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