Recombinant Arabidopsis thaliana Squamosa promoter-binding-like protein 9 (SPL9)

Code CSB-YP751202DOA
MSDS
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Source Yeast
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Code CSB-EP751202DOA
MSDS
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Source E.coli
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Code CSB-EP751202DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP751202DOA
MSDS
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Source Baculovirus
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Code CSB-MP751202DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
SPL9
Uniprot No.
Alternative Names
SPL9; At2g42200; T24P15.11; Squamosa promoter-binding-like protein 9
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-375
Target Protein Sequence
MEMGSNSGPG HGPGQAESGG SSTESSSFSG GLMFGQKIYF EDGGGGSGSS SSGGRSNRRV RGGGSGQSGQ IPRCQVEGCG MDLTNAKGYY SRHRVCGVHS KTPKVTVAGI EQRFCQQCSR FHQLPEFDLE KRSCRRRLAG HNERRRKPQP ASLSVLASRY GRIAPSLYEN GDAGMNGSFL GNQEIGWPSS RTLDTRVMRR PVSSPSWQIN PMNVFSQGSV GGGGTSFSSP EIMDTKLESY KGIGDSNCAL SLLSNPHQPH DNNNNNNNNN NNNNNTWRAS SGFGPMTVTM AQPPPAPSQH QYLNPPWVFK DNDNDMSPVL NLGRYTEPDN CQISSGTAMG EFELSDHHHQ SRRQYMEDEN TRAYDSSSHH TNWSL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Trans-acting factor that binds specifically to the consensus nucleotide sequence 5'-TNCGTACAA-3'.
Gene References into Functions
  1. Results suggest the mechanism by which miR156 and miR157 regulate gene expression of SQUAMOSA PROMOTER BINDING PROTEIN-LIKE (SPL) transcription factors SPL9 and SPL13. PMID: 29672610
  2. Analysis of plants carrying microRNA miR156-resistant forms of SQUAMOSA PROMOTER BINDING PROTEIN LIKE (SPL) genes showed that wall ingrowth deposition was increased in SPL9-group. PMID: 28082719
  3. Our results uncover a molecular mechanism for plant adaptation to the environment through the miR156-SPLs-DFR pathway, which coordinates development and abiotic stress tolerance. PMID: 25345491
  4. One of the miR156 targets, SPL9, negatively regulates anthocyanin accumulation. PMID: 21487097
  5. SPL9 has a role in shoot maturation. PMID: 18278578
  6. Data demonstrate that miR156 is a quantitative, rather than spatial, modulator of SPL9 expression in leaf primordia and that SPL9 activity nonautonomously inhibits initiation of new leaves at the shoot apical meristem. PMID: 18492871

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Subcellular Location
Nucleus. Cytoplasm. Note=Mostly located in nucleus.
Database Links

KEGG: ath:AT2G42200

STRING: 3702.AT2G42200.1

UniGene: At.498

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