Recombinant Arabidopsis thaliana Sucrose nonfermenting 4-like protein (SNF4)

Code CSB-YP856700DOA
MSDS
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Source Yeast
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Code CSB-EP856700DOA
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Source E.coli
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Code CSB-EP856700DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP856700DOA
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Source Baculovirus
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Code CSB-MP856700DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
SNF4
Uniprot No.
Alternative Names
SNF4; CBSCBS3; At1g09020; F7G19.11Sucrose nonfermenting 4-like protein; SNF4; CBS domain-containing protein CBSCBS3; SNF1-related protein kinase regulatory subunit betagamma; AKIN subunit betagamma; AKINbetagamma
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-487
Target Protein Sequence
MFGSTLDSSR GNSAASGQLL TPTRFVWPYG GRRVFLSGSF TRWTEHVPMS PLEGCPTVFQ VICNLTPGYH QYKFFVDGEW RHDEHQPFVS GNGGVVNTIF ITGPDMVPAG FSPETLGRSN MDVDDVFLRT ADPSQEAVPR MSGVDLELSR HRISVLLSTR TAYELLPESG KVIALDVNLP VKQAFHILYE QGIPLAPLWD FGKGQFVGVL GPLDFILILR ELGTHGSNLT EEELETHTIA AWKEGKAHIS RQYDGSGRPY PRPLVQVGPY DNLKDVALKI LQNKVAAVPV IYSSLQDGSY PQLLHLASLS GILKCICRYF RHSSSSLPIL QQPICSIPLG TWVPRIGESS SKPLATLRPH ASLGSALALL VQAEVSSIPV VDDNDSLIDI YSRSDITALA KDKAYAQIHL DDMTVHQALQ LGQDASPPYG IFNGQRCHMC LRSDSLVKVM ERLANPGVRR LVIVEAGSKR VEGIISLSDV FQFLLGL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Regulatory subunit of the probable trimeric SNF1-related protein kinase (SnRK) complex, which may play a role in a signal transduction cascade regulating gene expression and carbohydrate metabolism in higher plants.
Gene References into Functions
  1. hese results indicate that Arabidopsis KINbetagamma is critical for the regulation of ROS levels by mediating the biogenesis of mitochondria and peroxisomes in pollen, which is required for pollen-stigma interactions during pollination. PMID: 27472382
  2. AKINbetagamma interacts with other members of the AKIN complex in the two-hybrid system suggesting its involvement in heterotrimeric complexes located in both cytoplasm and nucleus. [AKINbetagamma] PMID: 17028154
Subcellular Location
Nucleus. Cytoplasm.
Protein Families
5'-AMP-activated protein kinase gamma subunit family
Tissue Specificity
Preferentially expressed in dividing cells, as well as in shoot apex and flower buds, but expressed at a lower level in differentiated tissues of roots, and leaves of rosette and inflorescence.
Database Links

KEGG: ath:AT1G09020

STRING: 3702.AT1G09020.1

UniGene: At.10386

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