Recombinant Arabidopsis thaliana Sulfite oxidase (SOX)

Code CSB-YP022954DOA
MSDS
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Source Yeast
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Code CSB-EP022954DOA
MSDS
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Source E.coli
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Code CSB-EP022954DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP022954DOA
MSDS
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Source Baculovirus
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Code CSB-MP022954DOA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
SOX
Uniprot No.
Alternative Names
SOX; MCP; At3g01910; F1C9.31; F28J7.38; Sulfite oxidase; EC 1.8.3.1; Moco-containing protein AtMCP; At-SO; AtSOX
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-393
Target Protein Sequence
MPGIRGPSEY SQEPPRHPSL KVNAKEPFNA EPPRSALVSS YVTPVDLFYK RNHGPIPIVD HLQSYSVTLT GLIQNPRKLF IKDIRSLPKY NVTATLQCAG NRRTAMSKVR NVRGVGWDVS AIGNAVWGGA KLADVLELVG IPKLTASTNL GARHVEFVSV DRCKEENGGP YKASITLSQA TNPEADVLLA YEMNGETLNR DHGFPLRVVV PGVIGARSVK WLDSINVIAE ESQGFFMQKD YKMFPPSVNW DNINWSSRRP QMDFPVQSAI CSVEDVQMVK PGKVSIKGYA VSGGGRGIER VDISLDGGKN WVEASRTQEP GKQYISEHSS SDKWAWVLFE ATIDVSQTTE VIAKAVDSAA NVQPENVESV WNLRGVLNTS WHRVLLRLGH SNL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Probably involved in sulfite oxidative detoxification.
Gene References into Functions
  1. the significance of sulfite oxidase for the overall shoot response to SO2 in relation to alterations in plant phenology and physiology PMID: 23025405
  2. In contrast to chicken sulfite oxidase (SO), Arabidopsis SO crystallizes without a sulfate bound in the active site. PMID: 22263579
  3. The purpose of the present study was to determine the subcellular localization of this novel plant enzyme. Immunogold electron-microscopic analysis showed the gold labels nearly exclusively in the peroxisomes. T PMID: 15653809
  4. structural analysis of the Mo(V) forms of sulfite oxidase from Arabidopsis thaliana PMID: 16201753
  5. Could be responsible for removing sulfite as a toxic metabolite, which might represent a means to protect the cell against excess of sulfite derived from SO2 gas in the atmosphere (acid rain) or during the decomposition of sulfur-containing amino acids. PMID: 16407262
  6. AtSO is expressed mainly in the green tissues/organs in a light-dependent way PMID: 17960039
  7. The isotropic hfi constant of 33S determined in these experiments was about 3 MHz, which demonstrates the presence of coordinated sulfate in the sulfite-reduced low-pH form of the plant enzyme. PMID: 17983221

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Subcellular Location
Peroxisome.
Database Links

KEGG: ath:AT3G01910

STRING: 3702.AT3G01910.1

UniGene: At.24506

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