Recombinant Arabidopsis thaliana Thiol protease aleurain (ALEU)

Code CSB-YP814115DOA
MSDS
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Source Yeast
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Code CSB-EP814115DOA
MSDS
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Source E.coli
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Code CSB-EP814115DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP814115DOA
MSDS
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Source Baculovirus
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Code CSB-MP814115DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ALEU
Uniprot No.
Alternative Names
ALEU; AALP; SAG2; At5g60360; MUF9.1; Thiol protease aleurain; AtALEU; EC 3.4.22.16; Senescence-associated gene product 2
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
141-358
Target Protein Sequence
LPETKDWRED GIVSPVKDQG GCGSCWTFST TGALEAAYHQ AFGKGISLSE QQLVDCAGAF NNYGCNGGLP SQAFEYIKSN GGLDTEKAYP YTGKDETCKF SAENVGVQVL NSVNITLGAE DELKHAVGLV RPVSIAFEVI HSFRLYKSGV YTDSHCGSTP MDVNHAVLAV GYGVEDGVPY WLIKNSWGAD WGDKGYFKME MGKNMCGIAT CASYPVVA
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
May play a role in proteolysis leading to mobilization of nitrogen during senescence and starvation.
Subcellular Location
Vacuole. Note=Predominantly vacuolar. From the Golgi apparatus, transported to the lytic vacuole (LV) in clathrin-coated vesicles (CCVs) via the prevacuolar compartment (PVC). In root elongating cells and stomata, localized in central vacuole and in small compartments (LVs, PVCs, Golgi bodies or small vacuoles). Limited to central vacuole in root elongated cells, leaf epidermal cells and trichomes of the lower face of the leaf blade. Limited to small compartments in root cap and apex cells, hypocotyl parenchyma cells and trichomes of the upper face of the leaf blade.
Protein Families
Peptidase C1 family
Tissue Specificity
Expressed in leaves (at protein level).
Database Links

KEGG: ath:AT5G60360

STRING: 3702.AT5G60360.3

UniGene: At.25414

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