Recombinant Arabidopsis thaliana Tocopherol cyclase, chloroplastic (VTE1)

Code CSB-YP856737DOA
MSDS
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Source Yeast
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Code CSB-EP856737DOA
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Source E.coli
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Code CSB-EP856737DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP856737DOA
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Source Baculovirus
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Code CSB-MP856737DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
VTE1
Uniprot No.
Alternative Names
VTE1; SXD1; At4g32770; F4D11.30; Tocopherol cyclase; chloroplastic; EC 5.5.1.24; Sucrose export defective 1; Vitamin E pathway gene 1 protein
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
77-488
Target Protein Sequence
TPHS GYHFDGTPRK FFEGWYFRVS IPEKRESFCF MYSVENPAFR QSLSPLEVAL YGPRFTGVGA QILGANDKYL CQYEQDSHNF WGDRHELVLG NTFSAVPGAK APNKEVPPEE FNRRVSEGFQ ATPFWHQGHI CDDGRTDYAE TVKSARWEYS TRPVYGWGDV GAKQKSTAGW PAAFPVFEPH WQICMAGGLS TGWIEWGGER FEFRDAPSYS EKNWGGGFPR KWFWVQCNVF EGATGEVALT AGGGLRQLPG LTETYENAAL VCVHYDGKMY EFVPWNGVVR WEMSPWGYWY ITAENENHVV ELEARTNEAG TPLRAPTTEV GLATACRDSC YGELKLQIWE RLYDGSKGKV ILETKSSMAA VEIGGGPWFG TWKGDTSNTP ELLKQALQVP LDLESALGLV PFFKPPGL
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Involved in the synthesis of both tocopherols and tocotrienols (vitamin E), which presumably protect photosynthetic complexes from oxidative stress. Catalyzes the conversion of 2-methyl-6-phytyl-1,4-hydroquinone and 2,3-dimethyl-5-phytyl-1,4-hydroquinone (DMPQ) to delta- and gamma-tocopherol respectively. Converts also 2,3-dimethyl-5-geranylgeranyl-1,4-hydroquinone (DMGQ) to gamma-tocotrienol.
Gene References into Functions
  1. ABC1K3 phosphorylates VTE1, possibly stabilizing it at plastoglobules. PMID: 23632854
  2. Evaluation of tocopherol cyclase over- and under-expression on metabolism of tocopheol, ascorbate, glutathione, and oxidative stress in A. thaliana transgenics and mutants. PMID: 15665245
  3. analysis of VTE1 localization and vitamin E accumulation in chloroplast plastoglobule lipoprotein particles PMID: 16414959
  4. Results show that both vte1 and vte4 mutant plant lines had higher concentration of protein carbonyl groups and oxidized glutathione compared to the wild type, indicating the development of oxidative stress. PMID: 19264498
Subcellular Location
Plastid, chloroplast, plastoglobule.
Database Links

KEGG: ath:AT4G32770

STRING: 3702.AT4G32770.1

UniGene: At.22259

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