Recombinant Arabidopsis thaliana Transcription factor CAULIFLOWER (CAL)

Code CSB-YP656497DOA
MSDS
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Source Yeast
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Code CSB-EP656497DOA
MSDS
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Source E.coli
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Code CSB-EP656497DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP656497DOA
MSDS
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Source Baculovirus
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Code CSB-MP656497DOA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CAL
Uniprot No.
Alternative Names
CAL; AGL10; At1g26310; F28B23.24Transcription factor CAULIFLOWER; AtCAL; Agamous-like MADS-box protein AGL10
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-255
Target Protein Sequence
MGRGRVELKR IENKINRQVT FSKRRTGLLK KAQEISVLCD AEVSLIVFSH KGKLFEYSSE SCMEKVLERY ERYSYAERQL IAPDSHVNAQ TNWSMEYSRL KAKIELLERN QRHYLGEELE PMSLKDLQNL EQQLETALKH IRSRKNQLMN ESLNHLQRKE KEIQEENSML TKQIKERENI LRTKQTQCEQ LNRSVDDVPQ PQPFQHPHLY MIAHQTSPFL NMGGLYQEED QTAMRRNNLD LTLEPIYNYL GCYAA
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Probable transcription factor that promotes early floral meristem identity in synergy with APETALA1, FRUITFULL and LEAFY. Is required subsequently for the transition of an inflorescence meristem into a floral meristem. Seems to be partially redundant to the function of APETALA1. Positively regulates the APETALA1 and LEAFY expression.
Gene References into Functions
  1. the differences between the Arabidopsis (Arabidopsis thaliana) APETALA1 (AP1) and CAULIFLOWER (CAL) duplicate genes in the time, space, and level of expression were determined by the presence or absence of functionally important transcription factor-binding sites (TFBSs) in regulatory regions. PMID: 27208240
  2. Expression of the transcription factor CAL is activated by LFY and the meristem identity regulator LMI1. PMID: 16554366
  3. The unique and redundant functions of the APETALA1 and CAULIFLOWER genes have been mapped to the four protein domains that characterize type-II MADS-domain proteins. PMID: 16893974
Subcellular Location
Nucleus.
Tissue Specificity
Expressed in young flower primordia.
Database Links

KEGG: ath:AT1G26310

STRING: 3702.AT1G26310.1

UniGene: At.41269

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