Recombinant Arabidopsis thaliana Transcription factor E2FB (E2FB)

Code CSB-YP887848DOA
MSDS
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Source Yeast
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Code CSB-EP887848DOA
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Source E.coli
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Code CSB-EP887848DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP887848DOA
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Source Baculovirus
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Code CSB-MP887848DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
E2FB
Uniprot No.
Alternative Names
E2FB; E2F1; At5g22220; T6G21; Transcription factor E2FB; E2F transcription factor-1; AtE2F1
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-469
Target Protein Sequence
MSEEVPQQFP SSKRQLHPSL SSMKPPLVAP GEYHRFDAAE TRGGGAVADQ VVSDAIVIKS TLKRKTDLVN QIVEVNELNT GVLQTPVSGK GGKAKKTSRS AKSNKSGTLA SGSNAGSPGN NFAQAGTCRY DSSLGLLTKK FINLIKQAED GILDLNKAAD TLEVQKRRIY DITNVLEGIG LIEKTLKNRI QWKGLDVSKP GETIESIANL QDEVQNLAAE EARLDDQIRE SQERLTSLSE DENNKRLLFV TENDIKNLPC FQNKTLIAVK APHGTTLEVP DPDEAGGYQR RYRIILRSTM GPIDVYLVSQ FEESFEDIPQ ADEPSNVPDE PSNVPDVPSN LPSTSGLPEN HDVSMPMKEE STERNMETQE VDDTQRVYSD IESHDFVDGI MKIVPPDLDM GVDYWFRSEV GEVSITDMWP DESGPDWNQM ITFDQDHAGP SDNKILEQPQ TPSSPTPEES TATRSPTGS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The binding of retinoblastoma-related proteins represses transactivation. Involved in the control of cell-cycle progression from G1 to S phase and from G2 to M phase. Stimulates cell proliferation and delays differentiation. Represses cell enlargement and endoreduplication in auxin-free conditions.
Gene References into Functions
  1. S6K1 interacts with retinoblastoma protein RBR via its N-terminal RBR binding motif, promotes its nuclear localization and consequent RBR-dependent repression of cell cycle genes through transcription factor E2FB. PMID: 23531690
  2. Results suggest that E2FB is one of the key targets for auxin to determine whether cells proliferate or whether they exit the cell cycle, enlarge, and endoreduplicate their DNA. PMID: 16055635
Subcellular Location
Cytoplasm. Nucleus. Note=Interaction with DPA induces an exclusive nuclear localization, but an interaction with DPB has no effect.
Protein Families
E2F/DP family
Tissue Specificity
Expressed in proliferating cells and several differentiated tissues. Detected in inflorescence and shoot apical meristems, cotyledonary vascular tissues, leaf primordia, young leaves, base of trichomes, central cylinder and elongation zone of roots, later
Database Links

KEGG: ath:AT5G22220

STRING: 3702.AT5G22220.2

UniGene: At.10189

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