Recombinant Arabidopsis thaliana Transcription factor FAMA (FMA)

Code CSB-YP690937DOA
MSDS
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Source Yeast
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Code CSB-EP690937DOA
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Source E.coli
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Code CSB-EP690937DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP690937DOA
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Source Baculovirus
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Code CSB-MP690937DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
FAMA
Uniprot No.
Alternative Names
FAMA; BHLH97; EN14; FMA; At3g24140; MUJ8.4Transcription factor FAMA; Basic helix-loop-helix protein 97; AtbHLH97; bHLH 97; Transcription factor EN 14; bHLH transcription factor bHLH097
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-414
Target Protein Sequence
MDKDYSAPNF LGESSGGNDD NSSGMIDYMF NRNLQQQQKQ SMPQQQQHQL SPSGFGATPF DKMNFSDVMQ FADFGSKLAL NQTRNQDDQE TGIDPVYFLK FPVLNDKIED HNQTQHLMPS HQTSQEGGEC GGNIGNVFLE EKEDQDDDND NNSVQLRFIG GEEEDRENKN VTKKEVKSKR KRARTSKTSE EVESQRMTHI AVERNRRKQM NEHLRVLRSL MPGSYVQRGD QASIIGGAIE FVRELEQLLQ CLESQKRRRI LGETGRDMTT TTTSSSSPIT TVANQAQPLI ITGNVTELEG GGGLREETAE NKSCLADVEV KLLGFDAMIK ILSRRRPGQL IKTIAALEDL HLSILHTNIT TMEQTVLYSF NVKITSETRF TAEDIASSIQ QIFSFIHANT NISGSSNLGN IVFT
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcription activator. Together with MYB88 and MYB124, ensures that stomata contain just two guard cells (GCs) by enforcing a single symmetric precursor cell division before stomatal maturity. Together with SPCH and MUTE, regulates the stomata formation. Required to promote differentiation and morphogenesis of stomatal guard cells and to halt proliferative divisions in their immediate precursors. Mediates the formation of stomata. Prevents histone H3K27me3 marks and derepresses stem cell gene expression.
Gene References into Functions
  1. Data indicate that loss of the basic helix-loop-helix transcription factor FAMA function abolishes myrosin idioblasts (MIs) fate as well as the expression of the myrosinase genes TGG1 and TGG2. PMID: 25304201
  2. Data indicate that the basic helix-loop-helix transcription factor FAMA as an essential component for myrosin cell development along leaf veins. PMID: 25304202
  3. Data indicate that MYB transcription factor FOUR LIPS (FLP) and bHLH transcription factor FAMA complement respective mutant phenotypes. PMID: 24571519
  4. Data indicate that bHLH transcription factor FAMA and MYB transcription factor FOUR LIPS (FLP) genes stabilize guard cell fate. PMID: 24654956
  5. Low humidity induces DNA methylation and transcription repression of SPCH and FAMA. PMID: 22442411
  6. Transcriptional changes in response to inducible expression of Arabidopsis FAMA, a basic helix-loop-helix protein whose actions during the final stage in stomatal development regulate both cell division and cell fate, is reported. PMID: 21245191

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Subcellular Location
Nucleus.
Tissue Specificity
Resctricted to stomatal cell lineages (at protein level). Expressed in roots, leaves, stems, and flowers.
Database Links

KEGG: ath:AT3G24140

STRING: 3702.AT3G24140.1

UniGene: At.37586

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