Recombinant Arabidopsis thaliana Transcription factor HFR1 (HFR1)

Code CSB-YP866899DOA
MSDS
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Source Yeast
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Code CSB-EP866899DOA
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Source E.coli
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Code CSB-EP866899DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP866899DOA
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Source Baculovirus
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Code CSB-MP866899DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
HFR1
Uniprot No.
Alternative Names
HFR1; BHLH26; EN68; FBI1; REP1; RSF1; At1g02340; T6A9.4; T6A9_13Transcription factor HFR1; Basic helix-loop-helix protein 26; AtbHLH26; bHLH 26; Protein LONG HYPOCOTYL IN FAR-RED 1; Protein REDUCED PHYTOCHROME SIGNALING; Reduced sensitivity to far-red light; Transcription factor EN 68; bHLH transcription factor bHLH026
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-292
Target Protein Sequence
MSNNQAFMEL GWRNDVGSLA VKDQGMMSER ARSDEDRLIN GLKWGYGYFD HDQTDNYLQI VPEIHKEVEN AKEDLLVVVP DEHSETDDHH HIKDFSERSD HRFYLRNKHE NPKKRRIQVL SSDDESEEFT REVPSVTRKG SKRRRRDEKM SNKMRKLQQL VPNCHKTDKV SVLDKTIEYM KNLQLQLQMM STVGVNPYFL PATLGFGMHN HMLTAMASAH GLNPANHMMP SPLIPALNWP LPPFTNISFP HSSSQSLFLT TSSPASSPQS LHGLVPYFPS FLDFSSHAMR RL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Atypical bHLH transcription factor that regulates photomorphogenesis through modulation of phytochrome (e.g. PHYA) and cryptochrome signalings (Ref.4, PubMed:11090209, PubMed:10995393, PubMed:19482971). Suppresses the transcriptional regulation activity of PIF4 by forming non-DNA-binding heterodimer.
Gene References into Functions
  1. light inactivates DET1, elevating HFR1 to sequester PIF1's suppression on seed germination PMID: 25775589
  2. The HLH and the adjacent C-terminal domain are required for biological activity of HFR1. PMID: 21205034
  3. OWL1 interacts with the basic helix-loop-helix HFR1 (LONG HYPOCOTYL IN FAR-RED) transcription factor. Both proteins are involved in the agravitropic response under far-red light. PMID: 19808946
Subcellular Location
Nucleus.
Tissue Specificity
Expressed constitutively in roots, leaves, stems, and flowers.
Database Links

KEGG: ath:AT1G02340

STRING: 3702.AT1G02340.1

UniGene: At.24795

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