Recombinant Arabidopsis thaliana Transcription factor HY5-like (HYH)

Code CSB-YP837789DOA
MSDS
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Source Yeast
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Code CSB-EP837789DOA
MSDS
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Source E.coli
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Code CSB-EP837789DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP837789DOA
MSDS
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Source Baculovirus
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Code CSB-MP837789DOA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
HYH
Uniprot No.
Alternative Names
HYH; BZIP64; At3g17609; MKP6.27Transcription factor HY5-like; HY5 homolog; bZIP transcription factor 64; AtbZIP64
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-149
Target Protein Sequence
MSLQRPNGNS SSSSSHKKHK TEESDEELLM VPDMEAAGST CVLSSSADDG VNNPELDQTQ NGVSTAKRRR GRNPVDKEYR SLKRLLRNRV SAQQARERKK VYVSDLESRA NELQNNNDQL EEKISTLTNE NTMLRKMLIN TRPKTDDNH
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Transcription factor that promotes photomorphogenesis in light. Acts downstream of the light receptor network and directly affects transcription of light-induced genes. Specifically involved in the blue light specific pathway, suggesting that it participates in transmission of cryptochromes (CRY1 and CRY2) signals to downstream responses. In darkness, its degradation prevents the activation of light-induced genes.
Gene References into Functions
  1. It has been reported that HY5 regulates HYH expression in roots and contributes to root growth under different light conditions. PMID: 28683099
  2. light affected TCP2 expression in a wavelength-dependent manner and TCP2 positively regulates mRNA expression of HYH and HY5. PMID: 26596765
  3. This study further reveals that MYC2 and GBF1 bind to HYH promoter and inhibit each other through non-DNA binding bHLH-bZIP heterodimers. PMID: 26047210
  4. The DNA binding activity of GBF1 is modulated by heterodimeriation with HY5 and HYH. PMID: 24157608
  5. BBX24 and BBX25 physically interact with HYH. PMID: 23733077
  6. the functional interrelations of GBF1 with HY5 and HYH in Arabidopsis seedling development. PMID: 22692212
  7. light triggers MSBP1 expression through direct binding to and activation by HY5 and HYH. PMID: 21715650
  8. HY5 and HYH are two necessary regulators that play a pivotal role during low temperature-induced anthocyanin accumulation in Arabidopsis seedlings. PMID: 20932601
  9. Together with HY5/HYH, gibberellins signaling may play an important role during low temperature-induced anthocyanin accumulation. PMID: 21636970
  10. HY5 and HYH were activators of NIA2, but inhibitors of NRT1.1 when tested across various light treatments and tissue types. PMID: 19540016
  11. The data support the hypothesis that the opposite root system phenotypes of hy5 single and hy5 hyh double mutants represent the morphological response to a quantitative gradient in the same molecular process. PMID: 17121469
  12. important for high nitrate reductase expression in red light PMID: 17929051
  13. This research provides evidence that, in ultraviolet B (UV-B) signaling, UVR8, HY5, and HYH act together and demonstrates a new role for HYH in UV-B responses. PMID: 18055587
  14. HYH and PEX11b constitute a novel branch of the phyA-mediated light signaling cascade, which promotes peroxisome proliferation during seedling photomorphogenesis. PMID: 18203870

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Subcellular Location
Nucleus.
Protein Families
BZIP family
Database Links

KEGG: ath:AT3G17609

STRING: 3702.AT3G17609.2

UniGene: At.28302

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