Recombinant Arabidopsis thaliana Transcription factor MYB29 (MYB29)

Code CSB-YP866975DOA
MSDS
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Source Yeast
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Code CSB-EP866975DOA
MSDS
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Source E.coli
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Code CSB-EP866975DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP866975DOA
MSDS
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Source Baculovirus
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Code CSB-MP866975DOA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
MYB29
Uniprot No.
Alternative Names
MYB29; HAG3; PMG2; At5g07690; MBK20.15; Transcription factor MYB29; Myb-related protein 29; AtMYB29; Protein HIGH ALIPHATIC GLUCOSINOLATE 3; Protein PRODUCTION OF METHIONINE-DERIVED GLUCOSINOLATE 2
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-336
Target Protein Sequence
MSRKPCCVGE GLKKGAWTAE EDKKLISYIH EHGEGGWRDI PQKAGLKRCG KSCRLRWANY LKPDIKRGEF SYEEEQIIIM LHASRGNKWS VIARHLPKRT DNEIKNYWNT HLKKLLIDKG IDPVTHKPLA YDSNPDEQSQ SGSISPKSLP PSSSKNVPEI TSSDETPKYD ASLSSKKRCF KRSSSTSKLL NKVAARASSM GTILGASIEG TLISSTPLSS CLNDDFSETS QFQMEEFDPF YQSSEHIIDH MKEDISINNS EYDFSQFLEQ FSNNEGEEAD NTGGGYNQDL LMSDVSSTSV DEDEMMQNIT GWSNYLLDHS DFNYDTSQDY DDKNFI
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Plays a minor rheostat role in aliphatic glucosinolates (GLSs) biosynthesis, mostly short chained. Together with MYB28/HAG1 and MYB76/HAG2, promotes aliphatic glucosinolate biosynthesis but represses indolic glucosinolate biosynthesis. Prevents insect performance (e.g. lepidopteran insect Mamestra brassicae) by promoting glucosinolates.
Gene References into Functions
  1. MYB28 expression was induced by sulfur deficiency, while the expression levels of MYB29 and MYB76 were positively correlated with sulfur concentration. PMID: 23792303
  2. MYB28 and MYB29 synergistically functioned in the glucose-induced biosynthesis of aliphatic glucosinolates, but MYB28 was predominant over MYB29. PMID: 23329848
  3. MYB76 is not dependent on MYB28 and MYB29 for induction of aliphatic glucosinolates. PMID: 20348214
  4. Myb29 presumably plays an accessory function for methyl jasmonate-mediated induction of a set of aliphatic glucosinolate biosynthetic genes. PMID: 17420480
  5. HAG1/MYB28, HAG2/MYB76 and HAG3/MYB29 reciprocally transactivated each other in the control of aliphatic glucosinolate biosynthesis and downregulated the expression of genes involved in the control of indolic glucosinolate biosynthesis. PMID: 18042203

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Subcellular Location
Nucleus.
Tissue Specificity
Expressed in both vegetative and generative organs. Mostly present in seedlings, inflorescences, roots and stems, and, to a lower extent, in leaves (in midvein and trichomes) and siliques.
Database Links

KEGG: ath:AT5G07690

STRING: 3702.AT5G07690.1

UniGene: At.9094

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