Recombinant Arabidopsis thaliana Transcription factor PIF7 (BHLH72)

Code CSB-YP704112DOA
MSDS
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Source Yeast
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Code CSB-EP704112DOA
MSDS
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Source E.coli
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Code CSB-EP704112DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP704112DOA
MSDS
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Source Baculovirus
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Code CSB-MP704112DOA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
BHLH72
Uniprot No.
Alternative Names
BHLH72; EN109; PIF7; At5g61270; MFB13.4; Transcription factor PIF7; Basic helix-loop-helix protein 72; AtbHLH72; bHLH 72; Phytochrome-interacting factor 7; Transcription factor EN 109; bHLH transcription factor bHLH072
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-366
Target Protein Sequence
MSNYGVKELT WENGQLTVHG LGDEVEPTTS NNPIWTQSLN GCETLESVVH QAALQQPSKF QLQSPNGPNH NYESKDGSCS RKRGYPQEMD RWFAVQEESH RVGHSVTASA SGTNMSWASF ESGRSLKTAR TGDRDYFRSG SETQDTEGDE QETRGEAGRS NGRRGRAAAI HNESERRRRD RINQRMRTLQ KLLPTASKAD KVSILDDVIE HLKQLQAQVQ FMSLRANLPQ QMMIPQLPPP QSVLSIQHQQ QQQQQQQQQQ QQQQQFQMSL LATMARMGMG GGGNGYGGLV PPPPPPPMMV PPMGNRDCTN GSSATLSDPY SAFFAQTMNM DLYNKMAAAI YRQQSDQTTK VNIGMPSSSS NHEKRD
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Transcription factor acting negatively in the phytochrome B signaling pathway under prolonged red light. Regulates PHYB abundance at the post-transcriptional level, possibly via the ubiquitin-proteasome pathway. May regulate the expression of a subset of genes by binding to the G-box motif.
Gene References into Functions
  1. PIF7 regulates the expression of shade-responsive genes.PIF7 recruits MRG1 and MRG2 that binds H3K4me3/H3K36me3 and brings histone-acetylases to induce histone acetylations to promote expression of shade responsive genes. PMID: 29187567
  2. New auxin synthesized through a PIF7-regulated pathway is required for shade-induced growth, linking directly the perception of a light quality signal to a rapid growth response. PMID: 22508725
  3. Data provide evidence that the mechanism by which PIF7 operate on the phyB signaling pathway under prolonged red light is through maintaining low phyB protein levels, in an additive or synergistic manner. PMID: 18252845
Subcellular Location
Nucleus. Note=Migrates rapidly to speckles upon light exposure.
Tissue Specificity
Expressed in flowers.
Database Links

KEGG: ath:AT5G61270

STRING: 3702.AT5G61270.1

UniGene: At.29114

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