Recombinant Arabidopsis thaliana Transcription factor TT2 (TT2)

Code CSB-YP880759DOA
MSDS
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Source Yeast
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Code CSB-EP880759DOA
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Source E.coli
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Code CSB-EP880759DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP880759DOA
MSDS
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Source Baculovirus
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Code CSB-MP880759DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
TT2
Uniprot No.
Alternative Names
TT2; MYB123; At5g35550; MOK9.18Transcription factor TT2; Myb-related protein 123; AtMYB123; Myb-related transcription factor LBM2-like; Protein TRANSPARENT TESTA 2
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-258
Target Protein Sequence
MGKRATTSVR REELNRGAWT DHEDKILRDY ITTHGEGKWS TLPNQAGLKR CGKSCRLRWK NYLRPGIKRG NISSDEEELI IRLHNLLGNR WSLIAGRLPG RTDNEIKNHW NSNLRKRLPK TQTKQPKRIK HSTNNENNVC VIRTKAIRCS KTLLFSDLSL QKKSSTSPLP LKEQEMDQGG SSLMGDLEFD FDRIHSEFHF PDLMDFDGLD CGNVTSLVSS NEILGELVPA QGNLDLNRPF TSCHHRGDDE DWLRDFTC
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcription activator, when associated with BHLH2/EGL3/MYC146, BHLH12/MYC1, or BHLH42/TT8. Involved in the control of flavonoid late metabolism in developing siliques. Plays a key role in determining the tissue-specific activation of leucoanthocyanidin reductase (BANYULS).
Gene References into Functions
  1. directly bound to the regulatory region of FUSCA3, and mediates the expression of numerous genes in the fatty acid biosynthesis pathway PMID: 24397827
  2. Loss of TT2 function results in more sufficient fatty acid synthesis. PMID: 22879396
  3. TT1 (TRANSPARENT TESTA1) interacts with the MYB transcription factor TT2. PMID: 21477081
  4. AtMYB5 and TT2, as partially redundant in regulating this outer seed coat developmental process with MYB5 having the major role. MYB5 and TT2 are shown to be expressed in this outer seed coat domain. PMID: 18992236
Subcellular Location
Nucleus.
Tissue Specificity
Expressed at a high level in immature siliques and at a lower level in flowers. Undetected in young seedlings, roots, leaves and inflorescence stems.
Database Links

KEGG: ath:AT5G35550

STRING: 3702.AT5G35550.1

UniGene: At.6888

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