Recombinant Arabidopsis thaliana Transcription factor bHLH54 (BHLH54)

Code CSB-YP818586DOA
MSDS
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Source Yeast
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Code CSB-EP818586DOA
MSDS
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Source E.coli
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Code CSB-EP818586DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP818586DOA
MSDS
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Source Baculovirus
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Code CSB-MP818586DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
BHLH54
Uniprot No.
Alternative Names
BHLH54; EN114; At1g27740; T22C5.19Transcription factor bHLH54; Basic helix-loop-helix protein 54; AtbHLH54; bHLH 54; Transcription factor EN 114; bHLH transcription factor bHLH054
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-258
Target Protein Sequence
MDVFVDGELE SLLGMFNFDQ CSSSKEERPR DELLGLSSLY NGHLHQHQHH NNVLSSDHHA FLLPDMFPFG AMPGGNLPAM LDSWDQSHHL QETSSLKRKL LDVENLCKTN SNCDVTRQEL AKSKKKQRVS SESNTVDESN TNWVDGQSLS NSSDDEKASV TSVKGKTRAT KGTATDPQSL YARKRREKIN ERLKTLQNLV PNGTKVDIST MLEEAVHYVK FLQLQIKLLS SDDLWMYAPL AYNGLDMGFH HNLLSRLM
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcription factor involved in the regulation of root hair elongation. Is sufficient to promote postmitotic cell growth in root-hair cells and is a direct transcriptional target of RHD6 and RSL1. Involved in the regulation of root hair elongation in response to low phosphate. Controls root hair cell growth by regulating the expression of genes encoding proteins involved in cell signaling, cell wall modification and secretion.
Gene References into Functions
  1. A trihelix transcription factor GT-2-LIKE1 (GTL1) and its homolog DF1 repress root hair growth. Transcriptional data, combined with genome-wide chromatin-binding data, show that GTL1 and DF1 directly bind the RSL4 promoter and regulate its expression to repress root hair growth. PMID: 29439132
  2. RSL4 controls cell growth by controlling the expression of genes encoding proteins involved in cell signalling, cell wall modification and secretion. PMID: 27452638
  3. Results suggest that RSL4 mediates root hair formation by regulating root hair-specific cis-element (RHE) genes and that this mechanism is conserved throughout the tracheophyte (vascular plant) lineage. PMID: 28087829
  4. study reports the discovery of a basic helix-loop-helix (bHLH) transcription factor called RSL4 (ROOT HAIR DEFECTIVE 6-LIKE 4) that is sufficient to promote postmitotic cell growth in Arabidopsis thaliana root-hair cells PMID: 20139979
Subcellular Location
Nucleus.
Tissue Specificity
Expressed constitutively in roots, leaves, and flowers. Expressed in root epidermal hair cells.
Database Links

KEGG: ath:AT1G27740

STRING: 3702.AT1G27740.1

UniGene: At.41015

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