Recombinant Arabidopsis thaliana Transcription factor bHLH83 (BHLH83)

Code CSB-YP887227DOA
MSDS
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Source Yeast
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Code CSB-EP887227DOA
MSDS
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Source E.coli
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Code CSB-EP887227DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP887227DOA
MSDS
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Source Baculovirus
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Code CSB-MP887227DOA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
BHLH83
Uniprot No.
Alternative Names
BHLH83; EN112; At1g66470; F28G11.9; Transcription factor bHLH83; Basic helix-loop-helix protein 83; AtbHLH83; bHLH 83; Transcription factor EN 112; bHLH transcription factor bHLH083
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-298
Target Protein Sequence
MALVNDHPNE TNYLSKQNSS SSEDLSSPGL DQPDAAYAGG GGGGGSASSS STMNSDHQQH QGFVFYPSGE DHHNSLMDFN GSSFLNFDHH ESFPPPAISC GGSSGGGGFS FLEGNNMSYG FTNWNHQHHM DIISPRSTET PQGQKDWLYS DSTVVTTGSR NESLSPKSAG NKRSHTGEST QPSKKLSSGV TGKTKPKPTT SPKDPQSLAA KNRRERISER LKILQELVPN GTKVDLVTML EKAISYVKFL QVQVKVLATD EFWPAQGGKA PDISQVKDAI DAILSSSQRD RNSNLITN
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcription factor that is specifically required for the development of root hairs. Acts with RSL1 to positively regulate root hair development. Acts downstream of genes that regulate epidermal pattern formation, such as GL2. Targets directly RSL4, another transcription factor involved in the regulation of root hair elongation. Acts with RSL1 as transcription factor that integrates a jasmonate (JA) signaling pathway that stimulates root hair growth.
Gene References into Functions
  1. S-nitrosoglutathione and auxin restored the root hair phenotype of the hairless root hair defective 6 (rhd6) mutant. PMID: 27880005
  2. data indicate that AtRHD6 (At1g66470) and AtRSL1 (At5g37800) are bHLH transcription factors that are specifically required for the development of root hairs and act downstream of the genes that regulate epidermal pattern formation PMID: 17556585
Subcellular Location
Nucleus.
Tissue Specificity
Expressed constitutively in flowers. Expressed in root epidermal hair cells.
Database Links

KEGG: ath:AT1G66470

STRING: 3702.AT1G66470.1

UniGene: At.35765

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