Recombinant Arabidopsis thaliana Transcription factor bHLH95 (BHLH95)

Code CSB-YP867030DOA
MSDS
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Source Yeast
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Code CSB-EP867030DOA
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Source E.coli
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Code CSB-EP867030DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP867030DOA
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Source Baculovirus
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Code CSB-MP867030DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
BHLH95
Uniprot No.
Alternative Names
BHLH95; EN21; RGE1; ZOU; At1g49770; F14J22.2Transcription factor bHLH95; Basic helix-loop-helix protein 95; AtbHLH95; bHLH 95; Protein RETARDED GROWTH OF EMBRYO 1; Protein ZHOUPI; Transcription factor EN 21; bHLH transcription factor bHLH095
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-308
Target Protein Sequence
MTNAQELGQE GFMWGISNSD DSGGGCKRIE KEPLPSHPSH PSPEIQTTTV KKGKKRTKRN DKNHEEESPD HEIHIWTERE RRKKMRDMFS KLHALLPQLP PKADKSTIVD EAVSSIKSLE QTLQKLEMQK LEKLQYSSAS TNTTPTTTFA YAPSSSSSPT ALLTPISNHP IDATATDSYP RAAFLADQVS SSSAAAANLP YPCNDPIVNF DTWSSRNVVL TICGNEAFFN LCVPKHKPGV FTSVCYLFEK YNMEVLFANV SSNVFWSTYV IQAQVNPSCE NQLLGNGLGV VDVFKQVSQE LVLYFSSL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcription factor that controls embryo growth. Regulates endosperm breakdown and embryonic epidermal development. Regulates the expression of SBT2.4/ALE1. Involved in embryonic cuticle formation upstream of SBT2.4/ALE1. Does not seem to be a general regulator of endosperm patterning. May control specific signaling pathways that coordinate embryo invasion and breakdown of surrounding endosperm tissues. Required for the production the embryo sheath, an extracuticular endosperm-derived structure at the surface of the embryo. Acts upstream of KRS.
Gene References into Functions
  1. ZHOUPI role in the eplant embryo/endosperm separation and the formation of the extracuticular sheath at the seed surface PMID: 28696222
  2. RED1 plays important roles in embryo development, seed maturation and plant growth. PMID: 27477025
  3. ZHOUPI regulates embryonic surface formation via a signaling pathway mediated by ABNORMAL LEAF SHAPE1. PMID: 23531691
  4. ZHOUPI controls embryonic cuticle formation via a signalling pathway involving the subtilisin protease ABNORMAL LEAF-SHAPE1 and the receptor kinases GASSHO1 and GASSHO2. PMID: 23318634
  5. RGE1 expression in the endosperm at the heart stage of embryo development plays an important role in controlling embryo growth. PMID: 18567831
  6. ZOU is not a general regulator of endosperm patterning, but rather controls specific signalling pathways that coordinate embryo invasion and breakdown of surrounding endosperm tissues. PMID: 18849529

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Subcellular Location
Nucleus.
Tissue Specificity
Siliques. Not detected in roots, rosette leaves or flowers. Expressed in endosperm cells.
Database Links

KEGG: ath:AT1G49770

STRING: 3702.AT1G49770.1

UniGene: At.38170

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