Recombinant Arabidopsis thaliana Triacylglycerol lipase SDP1 (SDP1), partial

Code CSB-YP881871DOA
MSDS
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Source Yeast
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Code CSB-EP881871DOA
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Source E.coli
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Code CSB-EP881871DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP881871DOA
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Source Baculovirus
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Code CSB-MP881871DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
SDP1
Uniprot No.
Alternative Names
SDP1; At5g04040; F8F6.10; F8F6.250; Triacylglycerol lipase SDP1; EC 3.1.1.3; Protein SUGAR-DEPENDENT 1
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Involved in the release of fatty acids from the oil body in germinating seedlings. Can hydrolyze triacylglycerols and diacylglycerols but not monoacylglycerols, phospholipids, galactolipids or cholesterol esters. SDP1 lipase activity is required to limit triacylglycerol accumulation in roots, leaves and stems, which are vegetative tissues. Functions synergistically with PDAT1 in regulating fatty acid flow from membrane lipid synthesis toward peroxisomal beta-oxidation through a transient triacylglycerol pool.
Gene References into Functions
  1. Data show that PHOSPHOLIPID:DIACYLGLYCEROL ACYLTRANSFERASE1 (PDAT1) and SUGAR-DEPENDENT1 (SDP1) TAG lipase, function synergistically in directing fatty acids (FAs) toward peroxisomal beta-oxidation via triacylglycerol (TAG) intermediates. PMID: 25293755
  2. Arabidopsis SDP1 lipase is delivered to oil bodies by peroxisome extensions PMID: 25775518
  3. SDP1 limits triacylglycerol accumulation in vegetative tissues of Arabidopsis. PMID: 23686420
  4. RNAi suppression during seed development results in a gain in oil yield PMID: 23171303
  5. SDP1 encodes a lipase that initiates oil breakdown in germinating Arabidopsis seeds. [SDP1] PMID: 16473965

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Subcellular Location
Lipid droplet. Membrane; Peripheral membrane protein. Peroxisome.
Tissue Specificity
Expressed in roots, leaves and stems.
Database Links

KEGG: ath:AT5G04040

STRING: 3702.AT5G04040.1

UniGene: At.24923

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