Recombinant Arabidopsis thaliana Two-component response regulator ARR22 (ARR22)

Code CSB-YP885553DOA
MSDS
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Source Yeast
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Code CSB-EP885553DOA
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Source E.coli
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Code CSB-EP885553DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP885553DOA
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Source Baculovirus
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Code CSB-MP885553DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ARR22
Uniprot No.
Alternative Names
ARR22; At3g04280; T6K12.10; Two-component response regulator ARR22
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-142
Target Protein Sequence
MATKSTGGTE KTKSIEVKKK LINVLIVDDD PLNRRLHEMI IKTIGGISQT AKNGEEAVIL HRDGEASFDL ILMDKEMPER DGVSTTKKLR EMKVTSMIVG VTSVADQEEE RKAFMEAGLN HCLEKPLTKA KIFPLISHLF DA
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Functions as response regulator involved in His-to-Asp phosphorelay signal transduction system. Phosphorylation of the Asp residue in the receiver domain activates the ability of the protein to promote the transcription of target genes. Type-A response regulators seem to act as negative regulators of the cytokinin signaling.
Gene References into Functions
  1. ARR22 inhibits gene transcription of an A-type response regulators upon cytokinin treatment in vivo. PMID: 26769709
  2. ARR22 plays a positive role in the stress tolerance response in part via enhancing cell membrane integrity and that phospho-histidine phosphatase activity of ARR22 may be required for this function. PMID: 24244460
  3. Spatial and temporal expression of ARR22 is discussed. PMID: 17099079
  4. Results favor the model that ARR22 acts as a phospho-histidine phosphatase on specific AHPs in the cytoplasm of Arabidopsis chalaza cells. PMID: 18625081
Subcellular Location
Nucleus.
Protein Families
ARR family, Type-A subfamily
Database Links

KEGG: ath:AT3G04280

STRING: 3702.AT3G04280.1

UniGene: At.40865

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