Recombinant Arabidopsis thaliana Two-component response regulator-like APRR1 (APRR1), partial

Code CSB-YP862812DOA
MSDS
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Source Yeast
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Code CSB-EP862812DOA
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Source E.coli
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Code CSB-EP862812DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP862812DOA
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Source Baculovirus
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Code CSB-MP862812DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
APRR1
Uniprot No.
Alternative Names
APRR1; AIP1; TOC1; At5g61380; MFB13.16Two-component response regulator-like APRR1; ABI3-interacting protein 1; Pseudo-response regulator 1; Timing of CAB expression 1
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Controls photoperiodic flowering response. Component of the circadian clock. Expression of several members of the ARR-like family is controlled by circadian rhythm. The particular coordinated sequential expression of APRR9, APRR7, APRR5, APRR3 and APPR1 result to circadian waves that may be at the basis of the endogenous circadian clock. Positive regulator of CCA1 and LHY expression.
Gene References into Functions
  1. TOC1 functions to provide a direct output from the core clock that transiently constrains the growth-promoting activity of the accumulating PIFs early postdusk, thereby gating growth to predawn, when conditions for cell elongation are optimal. PMID: 27071129
  2. Results show that TOC1 and CCA1 mRNA transcription levels are regulated by the newly identified Hesp gene and suggest a deadenylation as a mechanism involved in the regulation of the circadian clock. PMID: 26619288
  3. Rhythmic expression of TOC1 under light-dark cycling conditions correlates with histone modification. PMID: 22878891
  4. findings show that TOC1 does not function as an activator but rather as a general repressor of oscillator gene expression; repression occurs through TOC1 rhythmic association to the promoters of the oscillator genes PMID: 22403178
  5. These results define a biochemical action for the core clock protein TOC1 and refine our perspective on how plant clocks function. PMID: 22315425
  6. TOC1 rhythmic expression is affected by over-expression and mutation of RVE8/LCL5 mis-expressing plants. PMID: 21205033
  7. TOC1 and abscisic acid (ABA)-related gene overexpressing plants show defective responses to drought and support the notion that clock-dependent gating of ABA function is important for cellular homeostasis under dry environments. PMID: 19816401
  8. Lengthened circadian period alters expression of core oscillator genes, and limited degradation of TOC1. PMID: 18433436
  9. TOC1 plays a role in the photoperiodic control of flowering time and in the light-responsive control of elongation of hypocotyls. PMID: 19098071

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Subcellular Location
Nucleus.
Protein Families
ARR-like family
Tissue Specificity
Expressed in leaves, flowers and siliques. Restricted to the vasculature.
Database Links

KEGG: ath:AT5G61380

STRING: 3702.AT5G61380.1

UniGene: At.21919

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