Recombinant Arabidopsis thaliana UDP-glycosyltransferase 72E1 (UGT72E1)

Code CSB-YP842464DOA
MSDS
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Source Yeast
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Code CSB-EP842464DOA
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Source E.coli
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Code CSB-EP842464DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP842464DOA
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Source Baculovirus
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Code CSB-MP842464DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
UGT72E1
Uniprot No.
Alternative Names
UGT72E1; At3g50740; F18B3.20; UDP-glycosyltransferase 72E1; EC 2.4.1.-
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-487
Target Protein Sequence
MKITKPHVAM FASPGMGHII PVIELGKRLA GSHGFDVTIF VLETDAASAQ SQFLNSPGCD AALVDIVGLP TPDISGLVDP SAFFGIKLLV MMRETIPTIR SKIEEMQHKP TALIVDLFGL DAIPLGGEFN MLTYIFIASN ARFLAVALFF PTLDKDMEEE HIIKKQPMVM PGCEPVRFED TLETFLDPNS QLYREFVPFG SVFPTCDGII VNTWDDMEPK TLKSLQDPKL LGRIAGVPVY PIGPLSRPVD PSKTNHPVLD WLNKQPDESV LYISFGSGGS LSAKQLTELA WGLEMSQQRF VWVVRPPVDG SACSAYLSAN SGKIRDGTPD YLPEGFVSRT HERGFMVSSW APQAEILAHQ AVGGFLTHCG WNSILESVVG GVPMIAWPLF AEQMMNATLL NEELGVAVRS KKLPSEGVIT RAEIEALVRK IMVEEEGAEM RKKIKKLKET AAESLSCDGG VAHESLSRIA DESEHLLERV RCMARGA
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
UDP-glycosyltransferase that glucosylates coniferyl aldehyde to form coniferyl aldehyde 4-O-glucoside. Glucosylates sinapyl aldehyde to form sinapyl aldehyde 4-O-glucoside. Is not active in presence of coniferyl alcohol or sinapyl alcohol. Can glucosylate the phytotoxic xenobiotic compound 2,4,5-trichlorophenol (TCP).
Gene References into Functions
  1. SIS8, a putative MAPKKK, is a regulator of sugar response in Arabidopsis and interacts with a UDP-glucosyltransferase (UGT72E1) in the nucleus. PMID: 24320620
  2. over-expression of UGT72E1 in leaves led only to minimal changes in coniferyl alcohol 4-O-glucoside and no effect was observed on sinapoyl malate levels PMID: 18563437
Subcellular Location
Nucleus.
Protein Families
UDP-glycosyltransferase family
Tissue Specificity
Expressed in seedlings, roots and leaves.
Database Links

KEGG: ath:AT3G50740

STRING: 3702.AT3G50740.1

UniGene: At.20099

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