Recombinant Arabidopsis thaliana UDP-glycosyltransferase 78D3 (UGT78D3)

Code CSB-YP873259DOA
MSDS
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Source Yeast
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Code CSB-EP873259DOA
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Source E.coli
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Code CSB-EP873259DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP873259DOA
MSDS
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Source Baculovirus
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Code CSB-MP873259DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
UGT78D3
Uniprot No.
Alternative Names
UGT78D3; At5g17030; F2K13.180; UDP-glycosyltransferase 78D3; EC 2.4.1.-
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-459
Target Protein Sequence
MAKPSQPTRD SHVAVLVFPF GTHAAPLLAV TCRLATAAPS TVFSFFSTAR SNSSLLSSDI PTNIRVHNVD DGVPEGFVLT GNPQHAVELF LEAAPEIFRR EIKAAETEVG RKFKCILTDA FLWLAAETAA AEMKASWVAY YGGGATSLTA HLYTDAIREN VGVKEVGERM EETIGFISGM EKIRVKDTQE GVVFGNLDSV FSKTLHQMGL ALPRATAVFI NSFEELDPTF TNDFRSEFKR YLNIGPLALL SSPSQTSTLV HDPHGCLAWI EKRSTASVAY IAFGRVATPP PVELVAIAQG LESSKVPFVW SLQEMKMTHL PEGFLDRTRE QGMVVPWAPQ VELLNHEAMG VFVSHGGWNS VLESVSAGVP MICRPIFGDH AINARSVEAV WEIGVTISSG VFTKDGFEES LDRVLVQDDG KKMKVNAKKL EELAQEAVST KGSSFENFGG LLDEVVNFG
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Possesses low quercetin 3-O-glucosyltransferase activity in vitro.
Gene References into Functions
  1. First report on the construction of fusion proteins with expanded sugar-donor range and enhanced catalytic efficiencies for sugar donors. PMID: 23801300
  2. Data indicate that UGT, AtUGT78D3, has specificity for UDP-arabinose and uses quercetin as a sugar acceptor to make quercetin-3-O-arabinoside. PMID: 20981748
  3. Recombinant UGT78D3 (At5g17030) protein could convert quercetin to quercetin 3-O-arabinoside. The strict substrate specificity of UGT78D3 for flavonol aglycones and UDP-arabinose indicate that UGT78D3 is a flavonol arabinosyltransferase. PMID: 18757557
Protein Families
UDP-glycosyltransferase family
Database Links

KEGG: ath:AT5G17030

STRING: 3702.AT5G17030.1

UniGene: At.31606

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