Recombinant Arabidopsis thaliana Vacuolar-processing enzyme alpha-isozyme (At2g25940)

Code CSB-YP342980DOA
MSDS
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Source Yeast
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Code CSB-EP342980DOA
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Source E.coli
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Code CSB-EP342980DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP342980DOA
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Source Baculovirus
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Code CSB-MP342980DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
At2g25940
Uniprot No.
Alternative Names
At2g25940; T19L18.25; Vacuolar-processing enzyme alpha-isozyme; EC 3.4.22.34; Alpha-VPE; Asparaginyl endopeptidase alpha-VPE
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
21-478
Target Protein Sequence
DVIKLPSLAS KFFRPTENDD DSTKWAVLVA GSSGYWNYRH QADVCHAYQL LKKGGVKEEN IVVFMYDDIA KNEENPRPGV IINSPNGEDV YNGVPKDYTG DEVNVDNLLA VILGNKTALK GGSGKVVDSG PNDHIFIYYS DHGGPGVLGM PTSPNLYAND LNDVLKKKYA SGTYKSLVFY LEACESGSIF EGLLPEGLNI YATTASNAEE SSWGTYCPGE DPSPPSEYET CLGDLYSVAW IEDSEKHNLQ TETLHEQYEL VKKRTAGSGK SYGSHVMEFG DIGLSKEKLV LFMGTNPADE NFTFVNENSI RPPSRVTNQR DADLVHFWHK YQKAPEGSAR KVEAQKQVLE AMSHRLHVDN SILLIGILLF GLEGHAVLNK VRPSGEPLVD DWDCLKSLVR AFERHCGSLS QYGIKHMRSI ANMCNAGIQM RQMEEAAMQA CPTIPTSPWS SLDRGFSA
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Asparagine-specific endopeptidase involved in the processing of vacuolar seed protein precursors into the mature forms. Probably involved in post-translational proteolysis of seed storage proteins in the protein storage vacuole of developing seeds.
Subcellular Location
Vacuole.
Protein Families
Peptidase C13 family
Tissue Specificity
Specific to vegetative organs, especially in senescent tissues. Expressed in developing seeds, rosette leaves, cauline leaves and stems. Not expressed in the siliques. Also present at the branching points of the roots and in vascular tissues.
Database Links

KEGG: ath:AT2G25940

STRING: 3702.AT2G25940.1

UniGene: At.44999

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