Recombinant Arabidopsis thaliana WRKY transcription factor 6 (WRKY6), partial

Code CSB-YP880180DOA
MSDS
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Source Yeast
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Code CSB-EP880180DOA
MSDS
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Source E.coli
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Code CSB-EP880180DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP880180DOA
MSDS
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Source Baculovirus
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Code CSB-MP880180DOA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
WRKY6
Uniprot No.
Alternative Names
WRKY6; At1g62300; F19K23.22; WRKY transcription factor 6; WRKY DNA-binding protein 6; AtWRKY6
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcription factor involved in the control of processes related to senescence and pathogen defense. Interacts specifically with the W box (5'-(T)TGAC[CT]-3'), a frequently occurring elicitor-responsive cis-acting element. Activates the transcription of the SIRK gene and represses its own expression and that of the WRKY42 genes. Modulates phosphate homeostasis and Pi translocation by regulating PHO1 expression.
Gene References into Functions
  1. WRKY6 positively regulates dark-induced senescence and chlorophyll degradation.RGA physically interacts with WRKY6. PMID: 29574486
  2. WRKY6 restricts Piriformospora indica infection and phosphate-induced root development. PMID: 26718529
  3. Arabidopsis WRKY6 transcription factor played important roles in abscissic acid signaling by directly down-regulating RAV1 expression. PMID: 26829043
  4. WRKY40 and WRKY6 act downstream of the green leaf volatile E-2-hexenal.WRKY40 and WRKY6 mediate inhibition of root elongation by E-2-hexenal. PMID: 26243404
  5. WRKY6 is an arsenate-responsive transcription factor that mediates arsenate/phosphate transporter gene expression and restricts arsenate-induced transposon activation. PMID: 23922208
  6. Results established that WRKY6 is a low-B-induced transcription factor gene that is essential for normal root growth under low-B conditions. PMID: 20059736

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Subcellular Location
Nucleus.
Protein Families
WRKY group II-b family
Tissue Specificity
Roots, leaves, shoots, flowers and siliques.
Database Links

KEGG: ath:AT1G62300

STRING: 3702.AT1G62300.1

UniGene: At.25260

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