Recombinant Arabidopsis thaliana WUSCHEL-related homeobox 9 (WOX9)

Code CSB-YP744310DOA
MSDS
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Source Yeast
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Code CSB-EP744310DOA
MSDS
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Source E.coli
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Code CSB-EP744310DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP744310DOA
MSDS
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Source Baculovirus
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Code CSB-MP744310DOA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
WOX9
Uniprot No.
Alternative Names
WOX9; STIP; At2g33880; T1B8.31; WUSCHEL-related homeobox 9; Protein STIMPY
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-378
Target Protein Sequence
MASSNRHWPS MFKSKPHPHQ WQHDINSPLL PSASHRSSPF SSGCEVERSP EPKPRWNPKP EQIRILEAIF NSGMVNPPRE EIRRIRAQLQ EYGQVGDANV FYWFQNRKSR SKHKLRLLHN HSKHSLPQTQ PQPQPQPSAS SSSSSSSSSS KSTKPRKSKN KNNTNLSLGG SQMMGMFPPE PAFLFPVSTV GGFEGITVSS QLGFLSGDMI EQQKPAPTCT GLLLSEIMNG SVSYGTHHQQ HLSEKEVEEM RMKMLQQPQT QICYATTNHQ IASYNNNNNN NNIMLHIPPT TSTATTITTS HSLATVPSTS DQLQVQADAR IRVFINEMEL EVSSGPFNVR DAFGEEVVLI NSAGQPIVTD EYGVALHPLQ HGASYYLI
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Homeodomain transcription factor required for meristem growth and early development. Promotes cell proliferation and prevents premature differentiation in meristematic tissues during postembryonic development. Essential for maintaining tissue growth during embryogenesis. May act by repressing TSS to promote meristematic proliferation. Involved in the transcriptional activation of a subset of cytokinin response factors. May act as a negative regulator of cytokinin signaling in the dark.
Gene References into Functions
  1. Stip mutants show enhanced de-etiolation phenotype in response to cytokinin treatment in the dark, suggesting that STIP may be a negative regulator of cytokinin signaling under this condition. PMID: 21057190
  2. Expression of WOX8 is independent of the axis patterning signal auxin, but, together with the redundant gene WOX9, is activated in the zygote, its basal daughter cell, and the hypophysis by the zinc-finger transcription factor WRKY2. PMID: 21316593
  3. cytokinin signaling is involved in activation of the homeobox gene STIMPY (STIP or WOX9) expression in meristematic tissues, which is essential for maintaining the meristematic fate PMID: 20110319
  4. STIMPY (STIP; also called WOX9) is a homeobox gene required for the growth of the vegetative shoot apical meristem, in part by positively regulating WUSCHEL protein expression. [STIMPY] [STIP] [WOX9] PMID: 15753038
  5. Complete loss of STIP activity results in early embryonic arrest, most likely due to a failure in cell division. PMID: 17706632

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Subcellular Location
Nucleus. Cytoplasm.
Protein Families
WUS homeobox family
Tissue Specificity
Expressed in the basal cell and later at the boundary between suspensor and proembryo. Expressed at low levels in proliferating tissues post embryonically. Detected in vegetative shoot apical meristem, leaf primordia, floral meristems, emerging floral org
Database Links

KEGG: ath:AT2G33880

STRING: 3702.AT2G33880.1

UniGene: At.14699

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