Recombinant Arabidopsis thaliana Zinc finger protein CONSTANS-LIKE 1 (COL1)

Code CSB-YP527425DOA
MSDS
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Source Yeast
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Code CSB-EP527425DOA
MSDS
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Source E.coli
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Code CSB-EP527425DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP527425DOA
MSDS
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Source Baculovirus
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Code CSB-MP527425DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
COL1
Uniprot No.
Alternative Names
COL1; At5g15850; F14F8_230Zinc finger protein CONSTANS-LIKE 1
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-355
Target Protein Sequence
MLKVESNWAQ ACDTCRSAAC TVYCRADSAY LCSSCDAQVH AANRLASRHE RVRVCQSCER APAAFFCKAD AASLCTTCDS EIHSANPLAR RHQRVPILPI SEYSYSSTAT NHSCETTVTD PENRLVLGQE EEDEDEAEAA SWLLPNSGKN SGNNNGFSIG DEFLNLVDYS SSDKQFTDQS NQYQLDCNVP QRSYGEDGVV PLQIEVSKGM YQEQQNFQLS INCGSWGALR SSNGSLSHMV NVSSMDLGVV PESTTSDATV SNPRSPKAVT DQPPYPPAQM LSPRDREARV LRYREKKKMR KFEKTIRYAS RKAYAEKRPR IKGRFAKKKD VDEEANQAFS TMITFDTGYG IVPSF
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Putative transcription factor that may be involved in the light input to the circadian clock but does not affect flowering time.
Gene References into Functions
  1. MED25 links COI1 with HAC1-dependent H3K9 acetylation to activate MYC2-regulated transcription of jasmonic acid-responsive genes.COI1 Is enriched on the promoters of JAZ8 and ERF1. PMID: 28973940
  2. our data demonstrate a role for COI1 in disease resistance independent of Jasmonic Acid signaling and provide a molecular link between the JA and NB-LRR signaling pathways. PMID: 23093946
  3. The data demonstrate that ax6 enhances the effect of coi1 on JA responses, implying a genetic interaction between COI1 and AtCUL1 in JA signaling. PMID: 15860010
  4. Both COL1 and COR27 were found to be regulated by the circadian clock at warm growth temperatures. PMID: 19566593
Subcellular Location
Nucleus.
Protein Families
CONSTANS family
Tissue Specificity
Highly expressed in leaves and at lower levels in stems, flowers and siliques. Not detected in roots.
Database Links

KEGG: ath:AT5G15850

STRING: 3702.AT5G15850.1

UniGene: At.24486

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