Recombinant Autographa californica nuclear polyhedrosis virus Polyhedrin (PH)

Code CSB-YP361396ARA
MSDS
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Source Yeast
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Code CSB-EP361396ARA
MSDS
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Source E.coli
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Code CSB-EP361396ARA-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP361396ARA
MSDS
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Source Baculovirus
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Code CSB-MP361396ARA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
PH
Uniprot No.
Alternative Names
PH; P29; POLH; Polyhedrin; Major occlusion protein
Species
Autographa californica nuclear polyhedrosis virus (AcMNPV)
Expression Region
1-245
Target Protein Sequence
MPDYSYRPTI GRTYVYDNKY YKNLGAVIKN AKRKKHFAEH EIEEATLDPL DNYLVAEDPF LGPGKNQKLT LFKEIRNVKP DTMKLVVGWK GKEFYRETWT RFMEDSFPIV NDQEVMDVFL VVNMRPTRPN RCYKFLAQHA LRCDPDYVPH DVIRIVEPSW VGSNNEYRIS LAKKGGGCPI MNLHSEYTNS FEQFIDRVIW ENFYKPIVYI GTDSAEEEEI LLEVSLVFKV KEFAPDAPLF TGPAY
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Major component of the virus occlusion bodies which are large proteinaceous structures termed polyhedra. These structures serve as the protective package for the virus particles outside the infected host and allow natural transmission of virus between insect hosts, assisting persistence in the environment. Forms the paracrystalline lattice of polyhedra and interacts with enveloped virions as well as other accessory molecules and structures to form a mature viral occlusion body.
Gene References into Functions
  1. pk1 regulates polyhedrin promoter driven transcription of Autographa californica nucleopolyhedrovirus. PMID: 18353480
  2. A point mutation in polyhedrin is responsible for the mutant phenotype which resulted in the formation of few or no polyhedra and accumulation of a dispersed granular protein mass mainly in the cytoplasm of infected cells. PMID: 19038296
Subcellular Location
Host nucleus.
Protein Families
Polyhedrin family
Database Links

KEGG: vg:1403840

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