Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Uniprot No.

P03088

Research Area

others

Alternative Names

; Major capsid protein VP1; Major structural protein VP1

Species

BK polyomavirus (BKPyV)

Source

E.coli

Expression Region

1-362aa

Target Protein Sequence

MAPTKRKGECPGAAPKKPKEPVQVPKLLIKGGVEVLEVKTGVDAITEVECFLNPEMGDPDENLRGFSLKLSAENDFSSDSPERKMLPCYSTARIPLPNLNEDLTCGNLLMWEAVTVQTEVIGITSMLNLHAGSQKVHEHGGGKPIQGSNFHFFAVGGEPLEMQGVLMNYRSKYPDGTITPKNPTAQSQVMNTDHKAYLDKNNAYPVECWVPDPSRNENARYFGTFTGGENVPPVLHVTNTATTVLLDEQGVGPLCKADSLYVSAADICGLFTNSSGTQQWRGLARYFKIRLRKRSVKNPYPISFLLSDLINRRTQRVDGQPMYGMESQVEEVRVFDGTERLPGDPDMIRYIDKQGQLQTKML
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

45.1kDa

Protein Length

Full Length

Tag Info

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Producing recombinant BK polyomavirus major capsid protein VP1 involves several steps, starting with the isolation of the target gene, which corresponds to the full-length BK polyomavirus VP1. The gene is linked with an N-terminal 10xHis-tag and C-terminal Myc-tag gene and then cloned into an expression vector. The vector is introduced into E. coli cells via transformation. The positive cells are cultured for protein expression. The recombinant BK polyomavirus VP1 protein is harvested from the cell lysate. The protein is purified using affinity chromatography. Its purity is over 85% as determined by SDS-PAGE.

The BK polyomavirus (BKPyV) VP1 protein is the major capsid protein of the BKPyV and is important for the viral structure and function. BKPyV VP1 plays a significant role in host cell receptor recognition, proper virion assembly, and determining antigenicity and receptor specificity [1][2][3]. It is essential for the viability and growth of the BKPyV [2]. Studies have shown that VP1 can package DNA independently of the minor structural proteins VP2 and VP3, showcasing its ability to play a critical role in virus assembly [4].

References:
[1] J. Nilsson, N. Miyazaki, X. Li, B. Wu, L. Hammar, T. Liet al., Structure and assembly of virus-like particle in bk polyomavirus, Journal of Virology, vol. 79, no. 9, p. 5337-5345, 2005. https://doi.org/10.1128/jvi.79.9.5337-5345.2005
[2] A. Dugan, M. Gasparovic, N. Tsomaia, D. Mierke, B. O’Hara, K. Manleyet al., Identification of amino acid residues in bk virus vp1 that are critical for viability and growth, Journal of Virology, vol. 81, no. 21, p. 11798-11808, 2007. https://doi.org/10.1128/jvi.01316-07
[3] U. Neu, J. Wang, D. Macejak, R. Garcea, & T. Stehle, Structures of the major capsid proteins of the human karolinska institutet and washington university polyomaviruses, Journal of Virology, vol. 85, no. 14, p. 7384-7392, 2011. https://doi.org/10.1128/jvi.00382-11
[4] E. Gillock, S. Rottinghaus, D. Chang, X. Cai, S. Smiley, K. Anet al., Polyomavirus major capsid protein vp1 is capable of packaging cellular dna when expressed in the baculovirus system, Journal of Virology, vol. 71, no. 4, p. 2857-2865, 1997. https://doi.org/10.1128/jvi.71.4.2857-2865.1997

Target Background

Function

Forms an icosahedral capsid with a T=7 symmetry and a 50 nm diameter. The capsid is composed of 72 pentamers linked to each other by disulfide bonds and associated with VP2 or VP3 proteins. Interacts with gangliosides GT1b and GD1b containing terminal alpha(2-8)-linked sialic acids on the cell surface to provide virion attachment to target cell. This attachment induces virion internalization predominantly through caveolin-mediated endocytosis and traffics to the endoplasmic reticulum. Inside the endoplasmic reticulum, the protein folding machinery isomerizes VP1 interpentamer disulfide bonds, thereby triggering initial uncoating. Next, the virion uses the endoplasmic reticulum-associated degradation machinery to probably translocate in the cytosol before reaching the nucleus. Nuclear entry of the viral DNA involves the selective exposure and importin recognition of VP2/Vp3 nuclear localization signal. In late phase of infection, neo-synthesized VP1 encapsulates replicated genomic DNA in the nucleus, and participates in rearranging nucleosomes around the viral DNA.

Subcellular Location

Virion. Host nucleus.

Protein Families

Polyomaviruses coat protein VP1 family

Database Links

KEGG: vg:29031008

Code	CSB-EP360953BGYb1
Abbreviation	Recombinant BK polyomavirus Major capsid protein VP1 protein
MSDS	MSDS Datasheet
Size	US$388
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP360953BGYb1 could indicate that this peptide derived from E.coli-expressed BK polyomavirus (BKPyV) (Human polyomavirus 1) N/A. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP360953BGYb1 could indicate that this peptide derived from E.coli-expressed BK polyomavirus (BKPyV) (Human polyomavirus 1) N/A.
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Recombinant BK polyomavirus Major capsid protein VP1

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