Recombinant BK polyomavirus Major capsid protein VP1

Code CSB-YP360953BGY
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Source Yeast
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Code CSB-EP360953BGY-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP360953BGY
Size Pls inquire
Source Baculovirus
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Code CSB-MP360953BGY
Size Pls inquire
Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Uniprot No.
Alternative Names
; Major capsid protein VP1; Major structural protein VP1
Species
BK polyomavirus (BKPyV) (Human polyomavirus 1)
Expression Region
1-362
Target Protein Sequence
MAPTKRKGEC PGAAPKKPKE PVQVPKLLIK GGVEVLEVKT GVDAITEVEC FLNPEMGDPD ENLRGFSLKL SAENDFSSDS PERKMLPCYS TARIPLPNLN EDLTCGNLLM WEAVTVQTEV IGITSMLNLH AGSQKVHEHG GGKPIQGSNF HFFAVGGEPL EMQGVLMNYR SKYPDGTITP KNPTAQSQVM NTDHKAYLDK NNAYPVECWV PDPSRNENAR YFGTFTGGEN VPPVLHVTNT ATTVLLDEQG VGPLCKADSL YVSAADICGL FTNSSGTQQW RGLARYFKIR LRKRSVKNPY PISFLLSDLI NRRTQRVDGQ PMYGMESQVE EVRVFDGTER LPGDPDMIRY IDKQGQLQTK ML
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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 Q&A
Q:

What is the difference between CSB-MP360953BGY and CSB-MP360947BGY? Do they target different regions of the BK Large T antigen?

A:
Thanks for your inquiry. Regarding your requests, here are the detailed information.
1.Recombinant BK polyomavirus Major capsid protein VP1
CSB-YP360953BGY >> Yeast N-terminal 6xHis-tagged
CSB-EP360953BGY >> E.coli N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged In Stock.
CSB-EP360953BGYb1 >> E.coli N-terminal 10xHis-tagged and C-terminal Myc-tagged In Stock.
CSB-BP360953BGY >> Baculovirus N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged
CSB-MP360953BGY >> Mammalian cell N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged
Expression region: 1-362aa; Full length
Sequence:

MAPTKRKGECPGAAPKKPKEPVQVPKLLIKGGVEVLEVKTGVDAITEVECFLNPEMGDPDENLRGFSLKLSAENDFSSDSPERKMLPCYSTARIPLPNLNEDLTCGNLLMWEAVTVQTEVIGITSMLNLHAGSQKVHEHGGGKPIQGSNFHFFAVGGEPLEMQGVLMNYRSKYPDGTITPKNPTAQSQVMNTDHKAYLDKNNAYPVECWVPDPSRNENARYFGTFTGGENVPPVLHVTNTATTVLLDEQGVGPLCKADSLYVSAADICGLFTNSSGTQQWRGLARYFKIRLRKRSVKNPYPISFLLSDLINRRTQRVDGQPMYGMESQVEEVRVFDGTERLPGDPDMIRYIDKQGQLQTKML


2-1.Recombinant BK polyomavirus Large T antigen ,partial
CSB-YP360947BGY >> Yeast N-terminal 6xHis-tagged
CSB-EP360947BGY >> E.coli N-terminal 10xHis-tagged and C-terminal Myc-tagged
CSB-BP360947BGY >> Baculovirus N-terminal 10xHis-tagged and C-terminal Myc-tagged
CSB-MP360947BGY >> Mammalian cell N-terminal 10xHis-tagged and C-terminal Myc-tagged
Expression region: 1-133aa; Fragment at N-terminus
Sequence:

MDKVLNREESMELMDLLGLERAAWGNLPLMRKAYLRKCKEFHPDKGGDEDKMKRMNTLYKKMEQDVKVAHQPDFGTWSSSEVPTYGTEEWESWWSSFNEKWDEDLFCHEDMFASDEEATADSQHSTPPKKKRK
2-2.Recombinant BK polyomavirus Large T antigen ,partial
CSB-YP360947BGY1 >> Yeast N-terminal 6xHis-tagged
CSB-EP360947BGY1 >> E.coli N-terminal 10xHis-tagged and C-terminal Myc-tagged
CSB-BP360947BGY1 >> Baculovirus N-terminal 10xHis-tagged and C-terminal Myc-tagged
CSB-MP360947BGY1 >> Mammalian cell N-terminal 10xHis-tagged and C-terminal Myc-tagged
Expression region: 127 - 261aa; Partial protein
Sequence:

PPKKKRKVEDPKDFPSDLHQFLSQAVFSNRTLACFAVYTTKEKAQILYKKLMEKYSVTFISRHMCAGHNIIFFLTPHRHRVSAINNFCQK
LCTFSFLICKGVNKEYLLYSALTRDPYHTIEESIQGGLKEHDFSP


2-3.Recombinant BK polyomavirus Large T antigen ,partial
CSB-YP360947BGY2 >> Yeast N-terminal 6xHis-tagged
CSB-EP360947BGY2 >> E.coli N-terminal 10xHis-tagged and C-terminal Myc-tagged
CSB-BP360947BGY2 >> Baculovirus N-terminal 10xHis-tagged and C-terminal Myc-tagged
CSB-MP360947BGY2 >> Mammalian cell N-terminal 10xHis-tagged and C-terminal Myc-tagged
Expression region: 262 - 695aa; Fragment at C-terminus
Sequence:

EEPEETKQVSWKLITEYAVETKCEDVFLLLGMYLEFQYNVEECKKCQKKDQPYHFKYHEKHFANAIIFAESKNQKSICQQAVDTVLAKKRVDTLHMTREEMLTERFNHILDKMDLIFGAHGNAVLEQYMAGVAWLHCLLPKMDSVIFDFLHCIVFNVPKRRYWLFKGPIDSGKTTLAAGLLDLCGGKALNVNLPMERLTFELGVAIDQYMVVFEDVKGTGAESKDLPSGHGINNLDSLRDYLDGSVKVNLEKKHLNKRTQIFPPGLVTMNEYPVPKTLQARFVRQIDFRPKIYLRKSLQNSEFLLEKRILQSGMTLLLLLIWFRPVADFATDIQSRIVEWKERLDSEISMYTFSRMKYNICMGKCILDITREEDSETEDSGHGSSTESQSQCSSQVSDTSAPAEDSQRSDPHSQELHLCKGFQCFKRPKTPPPK

Target Background

Function
Forms an icosahedral capsid with a T=7 symmetry and a 50 nm diameter. The capsid is composed of 72 pentamers linked to each other by disulfide bonds and associated with VP2 or VP3 proteins. Interacts with gangliosides GT1b and GD1b containing terminal alpha(2-8)-linked sialic acids on the cell surface to provide virion attachment to target cell. This attachment induces virion internalization predominantly through caveolin-mediated endocytosis and traffics to the endoplasmic reticulum. Inside the endoplasmic reticulum, the protein folding machinery isomerizes VP1 interpentamer disulfide bonds, thereby triggering initial uncoating. Next, the virion uses the endoplasmic reticulum-associated degradation machinery to probably translocate in the cytosol before reaching the nucleus. Nuclear entry of the viral DNA involves the selective exposure and importin recognition of VP2/Vp3 nuclear localization signal. In late phase of infection, neo-synthesized VP1 encapsulates replicated genomic DNA in the nucleus, and participates in rearranging nucleosomes around the viral DNA.
Subcellular Location
Virion. Host nucleus.
Protein Families
Polyomaviruses coat protein VP1 family
Database Links

KEGG: vg:29031008

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