Recombinant Bacillus subtilis Probable flavodoxin-2 (ykuP)

Code CSB-EP518499BRJ
Size US$388
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Target Names
ykuP
Uniprot No.
Research Area
Others
Alternative Names
ykuP; BSU14170; Probable flavodoxin 2
Species
Bacillus subtilis (strain 168)
Source
E.coli
Expression Region
1-151aa
Target Protein Sequence
MAKILLVYATMSGNTEAMADLIEKGLQEALAEVDRFEAMDIDDAQLFTDYDHVIMGTYTWGDGDLPDEFLDLVEDMEEIDFSGKTCAVFGSGDTAYEFFCGAVDTLEAKIKERGGDIVLPSVKIENNPEGEEEEELINFGRQFAKKSGCAV
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
32.6kDa
Protein Length
Full Length
Tag Info
N-terminal 6xHis-SUMO-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Low-potential electron donor to a number of redox enzymes.
Gene References into Functions
  1. Ability of YkuP to transfer electrons toward cytochrome P450 monooxygenase CYP109B1 (Gene ID 936452)from B. subtilis is reproted PMID: 20186410
  2. Flavodoxins YkuP and YkuN from B. subtilis are shown to support oxidizing activity of cytochrome P450 monooxygenase CYP109B1 (GeneBanK CAB13078) from same organism. PMID: 20186410
  3. Interaction of YkuP with cytochrome P450 monooxygenase CYP109B1 from Bacillus subtilis 168 is reported. PMID: 20186410
  4. Cloning, expression, purification, and characterization of the flavin mononucleotide(FMN)-binding flavodoxin YkuP is reported. PMID: 15449930
Protein Families
Flavodoxin family
Database Links
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