Recombinant Bardet-Biedl syndrome 4 protein homolog (F58A4.14)

Code CSB-YP682589CXY
MSDS
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Source Yeast
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Code CSB-EP682589CXY
MSDS
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Source E.coli
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Code CSB-EP682589CXY-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP682589CXY
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP682589CXY
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
bbs-4
Uniprot No.
Alternative Names
bbs-4; F58A4.14Bardet-Biedl syndrome 4 protein homolog
Species
Caenorhabditis elegans
Expression Region
1-462
Target Protein Sequence
MEASNQDEII GTDVIPNEQD NPEEVVPEPT SLDVPPPPPE RAPSAPKRVE ILDCNSLNGL MYHYFAQGDY IECKSIIGEI QSKYLERNEA AFHVRGLIAR NEGELEEAME CFHKAYELSG KNKRYFYETG RCNFLLGRHQ IAVEQLTKAS EVMKDNPKVW YWLARAIYHF PAEKVQGKTF NPVESARTIL MKPDIAKDAT LICFLGRLCE ELGDTSGAIA AYKSSLKLQP DNTEVMNLLG LIYLRTGQVQ EGFVQLGNCL AYDPANSQAI LTIGSIMQNH SDHDVALNKY RVAADVSDYN GCLWNNIGIG LLARNKPAAS HSALKKAAFI NPLNYKISYN LGVLHDIMNL HCSALHYIKL CTELYPQNAK AVGAMAVILS HMNDDKNARL AYKKSIELKK NPSTILNYAI FEYRMKDVTA ASNALKLYKD LQASGVKCSA NNKETANLLE SVLKQPDQSA EQ
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Component of the BBSome complex. The BBSome complex is thought to function as a coat complex required for sorting of specific membrane proteins to the primary cilia. The BBSome complex is required for ciliogenesis but is dispensable for centriolar satellite function. Required for proper BBSome complex assembly and its ciliary localization. May be required for microtubule anchoring at the centrosome but not for microtubule nucleation. May be required for the dynein-mediated transport of pericentriolar proteins to the centrosome. Required, redundantly with bbs-5, for cilia biogenesis and both the assembly and movement of intraflagellar transport proteins along the ciliary axoneme. Plays a role in the removal of degraded mechanosensory receptors within the cilia.
Subcellular Location
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasm, cytoskeleton. Cell projection, cilium membrane.
Protein Families
BBS4 family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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