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Recombinant Beet necrotic yellow vein virus Protein P25

Recombinant Beet necrotic yellow vein virus Protein P25

Code	CSB-YP714596BSO
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Yeast
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Code	CSB-EP714596BSO
MSDS	MSDS Datasheet
Size	Pls inquire
Source	E.coli
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Code	CSB-EP714596BSO-B
MSDS	MSDS Datasheet
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP714596BSO
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP714596BSO
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Mammalian cell
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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Purity

≥85% (SDS-PAGE)

Uniprot No.

Q65669

Alternative Names

Protein P25; 25kDa protein

Species

Beet necrotic yellow vein virus (isolate Japan/S) (BNYVV)

Expression Region

1-219

Target Protein Sequence

MGDILGAVYD LGHRPYLARR TVYEDRLILS THGNVCRAIN LLTHDNRTTL VYHNNTKRIR FRGLLCAYRV PYCGFRALCR VMLCSLPRLC DIPINGSRDF VADPTRLDSS VNELLVSNGL VTHYDRVHNV PIHTDGFEVV DFTTVFRGPG NFLLPNATNF PRSTTTDQVY MVCLVNTVNC VLRFESELVV WVHSGLYAGD VLDVDNNVIQ APDGVDDND

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

■ Customer Reviews

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Target Background

Function

Pathogenicity factor implicated in symptom exacerbation. Might function as transcription activator (Potential).

Gene References into Functions

Expression of BNYVV p25 protein induces hormonal changes and a root branching phenotype in Arabidopsis thaliana. PMID: 20602166
Sequence analysis of natural BNYVV isolates and site-directed mutagenesis of the p25 protein revealed that 3 aa residues at positions 68, 70 and 179 are important in determining the resistance phenotype PMID: 18420811
A single U/C nucleotide substitution changing alanine to valine in the BNYV P25 protein promotes increased virus accumulation in roots of mechanically inoculated, partially resistant sugar beet seedlings. PMID: 19218223

Subcellular Location

Host cytoplasm. Host nucleus. Note=Shuttles between cytoplasm and nucleus.

Protein Families

Benyvirus P25 protein family

Database Links

KEGG: vg:991089

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