Recombinant Bovine Growth/differentiation factor 9(GDF9)

Code CSB-YP863896BO
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Source Yeast
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Code CSB-EP863896BO
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Source E.coli
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Code CSB-EP863896BO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP863896BO
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Source Baculovirus
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Code CSB-MP863896BO
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names GDF9
Uniprot No. Q9GK68
Alternative Names GDF9Growth/differentiation factor 9; GDF-9
Species Bos taurus (Bovine)
Expression Region 319-453
Target Protein Sequence DQ ESVSSELKKP LVPASFNLSE YFKQFLFPQN ECELHDFRLS FSQLKWDNWI VAPHKYNPRY CKGDCPRAVG HRYGSPVHTM VMNIIHEKLD SSVPRPSCVP AKYSPLSVLA IEPDGSIAYK EYEDMIATKC TCR
Protein Length Full Length of Mature Protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Data

Function Required for ovarian folliculogenesis.
Gene References into Functions
  1. This study concluded that the improved pregnancy rate after porcine LH-induced ovulation reported previously occurred through greater activation of intrafollicular transforming growth factor-beta1 superfamily members, as these proteins promote cumulus expansion and oocyte competence. PMID: 26794084
  2. Genetic variability is an important component of the number of antral follicles in the bovine ovary. PMID: 22959639
  3. Immunization against GDF9 and BMP15, alone or together, altered follicular development and ovulation rate in cattle. PMID: 19439562
  4. GDF9 expression in the oocytes of calf ovaries was significantly higher than in those of the adult ovaries PMID: 21401961
  5. GDF-9 mediates its effect through the pathway commonly activated by TGF beta and activin, but not that activated by many BMPs PMID: 12574210
  6. Maternal GDF9 transcripts persisted during oocyte in vitro maturation and fertilization and in preimplantation embryo until the five- to eight-cell or morula stage, but transcription was not reactivated at the time of embryonic genome activation. PMID: 15189828
  7. Oocyte- and granulosa cell-derived GDF9 may simultaneously promote theca cell proliferation and prevent premature differentiation of the theca interna during early follicle development. PMID: 17959852

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Subcellular Location Secreted
Protein Families TGF-beta family
Database Links

KEGG: bta:282574

STRING: 9913.ENSBTAP00000012476

UniGene: Bt.7047

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