Recombinant Bovine Superoxide dismutase [Mn], mitochondrial(SOD2)

Code CSB-YP022398BO
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Source Yeast
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Code CSB-EP022398BO
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Source E.coli
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Code CSB-EP022398BO-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP022398BO
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Source Baculovirus
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Code CSB-MP022398BO
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Source Mammalian cell
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Product Details

Purity >85% (SDS-PAGE)
Target Names SOD2
Uniprot No. P41976
Alternative Names SOD2; Superoxide dismutase [Mn]; mitochondrial; EC 1.15.1.1
Species Bos taurus (Bovine)
Expression Region 25-222
Target Protein Sequence KHSLPD LPYDYGALEP HINAQIMQLH HSKHHAAYVN NLNVAEEKYR EALEKGDVTA QIALQPALKF NGGGHINHSI FWTNLSPNGG GEPQGELLEA IKRDFGSFAK FKEKLTAVSV GVQGSGWGWL GFNKEQGRLQ IAACSNQDPL QGTTGLIPLL GIDVWEHAYY LQYKNVRPDY LKAIWNVINW ENVTARYTAC SK
Protein Length Full Length of Mature Protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.

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Target Background

Function
(From Uniprot)
Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.
Gene References into Functions
  1. Exposure to follicular fluid transiently increased the transcript levels of IL8 and PTGS2, and decreased the expression of SOD2, GPX3, DAB2, and NR3C1. TNF and IL6 levels were also decreased while those of NAMPT were unaffected. PMID: 24186266
  2. Data suggest that during ectogenesis of blastocysts infected with bovine Herpesvirus type 5, expression of SOD2 (Mn) remains high indicating intense mitochondrial activity; this effect may be involved in inhibition of apoptosis in infected embryos. PMID: 22823939
  3. expression profile of SOD2 in follicles: oocytes (SOD2 restricted to ooplasm); cumulus cells (expressed some SOD2); follicular fluid (small follicles show increased amounts of SOD2 in comparison with large follicles) PMID: 20197373
  4. Shear stress influences spatial variations in vascular Mn-SOD expression with implications for LDL nitration. PMID: 18434620
  5. The expression of SOD1 and SOD2 through the course of the estrous cycle is reported. PMID: 18572235
  6. Heart mitochondrial nucleoids contained SOD2, aortic endothelial cell mitochondrial nucleoids did not PMID: 19228881

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Subcellular Location Mitochondrion matrix.
Protein Families Iron/manganese superoxide dismutase family
Database Links

KEGG: bta:104973000

STRING: 9913.ENSBTAP00000008569

UniGene: Bt.4748

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