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Recombinant Burkholderia pseudomallei Translocator protein BipD (bipD)

Recombinant Burkholderia pseudomallei Translocator protein BipD (bipD)

Code	CSB-YP734020BXW
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Yeast
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Code	CSB-EP734020BXW
MSDS	MSDS Datasheet
Size	Pls inquire
Source	E.coli
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Code	CSB-EP734020BXW-B
MSDS	MSDS Datasheet
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP734020BXW
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP734020BXW
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Mammalian cell
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Product Details
Customer Reviews and Q&A
Target Background

Product Details

Purity

≥85% (SDS-PAGE)

Target Names

bipD

Uniprot No.

Q63K37

Alternative Names

bipD; BPSS1529Translocator protein BipD

Species

Burkholderia pseudomallei (strain K96243)

Expression Region

1-310

Target Protein Sequence

MNMHVDMGRA LTVRDWPALE ALAKTMPADA GARAMTDDDL RAAGVDRRVP EQKLGAAIDE FASLRLPDRI DGRFVDGRRA NLTVFDDARV AVRGHARAQR NLLERLETEL LGGTLDTAGD EGGIQPDPIL QGLVDVIGQG KSDIDAYATI VEGLTKYFQS VADVMSKLQD YISAKDDKNM KIDGGKIKAL IQQVIDHLPT MQLPKGADIA RWRKELGDAV SISDSGVVTI NPDKLIKMRD SLPPDGTVWD TARYQAWNTA FSGQKDNIQN DVQTLVEKYS HQNSNFDNLV KVLSGAISTL TDTAKSYLQI

Protein Length

full length protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

■ Customer Reviews

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Target Background

Function

Required for invasion of epithelial cells, as well as for survival within host cells, escape from endocytic vesicles and subsequent actin-tail formation. Probably regulates the secretion of effectors BipB and BipC and their final integration into the target cell membrane.

Gene References into Functions

BipD has been crystallized in a monoclinic crystal form that diffracts X-rays to 2.5 angstroms resolution PMID: 16880550
Residues 10-310 of mature BipD, a component of B. pseudomallei type III secretion system, has been crystallized. Native BipD crystals have undergone preliminary crystallographic analysis. PMID: 16946464
The crystal structure og BipD suggests that the biologically active form of the molecule may be a dimer formed by contacts involving the C-terminal alpha-helix, which is the most strongly conserved part of the protein. PMID: 16950399
The role of the N-terminal domain of BipD, a type III secretion system needle tip protein, is to chaperone residues on the coiled-coil involved in interactions at the tip of the needle. PMID: 17077085

Subcellular Location

Secreted. Note=Secreted via the bsa type III secretion system. Localizes to the tip of the external secretion needle that is part of the secretion apparatus.

Protein Families

Invasin protein D family

Database Links

KEGG: bps:BPSS1529

STRING: 272560.BPSS1529

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