Recombinant Chlamydophila psittaci Major outer membrane porin (ompA)

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Code CSB-EP310272CJB
Size US$306
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

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Product Details

Purity
Greater than 85% as determined by SDS-PAGE.
Target Names
ompA
Uniprot No.
Research Area
Microbiology
Alternative Names
ompA; omp1; Major outer membrane porin; MOMP
Species
Chlamydophila psittaci (Chlamydia psittaci)
Source
E.coli
Expression Region
23-392aa
Target Protein Sequence
LPVGNPAEPSLLIDGTMWEGASGDPCDPCATWCDAISIRAGFYGDYVFDRILKVDVNKTISGMAAAPTAASGTASNTTVAADRSNFAYGKHLQDAEWCTNAAYLALNIWDRFDVFCTLGASNGYFKASSDAFNLVGLIGLAGTDFANQRPNVEISQGIVELYTDTAFSWSVGARGALWECGCATLGAEFQYAQSNPKIEMLNVTSSPAQFMIHKPRGYKGTAANFPLPVAAGTATATDTKSATVKYHEWQVGLALSYRLNMLVPYIGVNWSRATFDADTIRIAQPKLASAILNLTTWNPTLLGVATTLDTSNKYADFMQIVSMQINKMKSRKACGIAVGATLIDADKWSITGEARLIDERAAHINAQFRF
Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request.
Mol. Weight
45.4 kDa
Protein Length
Full Length of Mature Protein
Tag Info
N-terminal 10xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
3-7 business days
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.
Description

Constructing a plasmid that codes for the Chlamydophila psittaci (Chlamydia psittaci) ompA protein (23-392aa) initiates the generation of the recombinant Chlamydophila psittaci (Chlamydia psittaci) ompA protein. The constructed plasmid is transformed into 23-392aa cells. Positive e.coli cells are selected and then cultured under conditions that encourage the expression of the gene of interest. The protein is equipped with a N-terminal 10xHis tag. After that, affinity purification is employed to isolate and purify the recombinant ompA protein from the cell lysate. The purity of the resulting recombinant ompA protein is over 85%, determined by SDS-PAGE.

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Target Background

Function
In elementary bodies (EBs, the infectious stage, which is able to survive outside the host cell) provides the structural integrity of the outer envelope through disulfide cross-links with the small cysteine-rich protein and the large cysteine-rich periplasmic protein. It has been described in publications as the Sarkosyl-insoluble COMC (Chlamydia outer membrane complex), and serves as the functional equivalent of peptidoglycan.; Permits diffusion of specific solutes through the outer membrane.
Subcellular Location
Cell outer membrane; Multi-pass membrane protein.
Protein Families
Chlamydial porin (CP) (TC 1.B.2) family
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