Recombinant Chondroitin synthase (kfoC)

Code CSB-YP817141ENL
MSDS
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Source Yeast
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Code CSB-EP817141ENL
MSDS
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Source E.coli
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Code CSB-EP817141ENL-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP817141ENL
MSDS
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Source Baculovirus
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Code CSB-MP817141ENL
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
kfoC
Uniprot No.
Alternative Names
kfoCChondroitin synthase; CS; Chondroitin polymerase) [Includes: Glucuronosyl-N-acetylgalactosaminyl-proteoglycan 4-beta-N-acetylgalactosaminyltransferase; EC 2.4.1.175; UDP-GalNAc transferase); N-acetylgalactosaminyl-proteoglycan 3-beta-glucuronosyltransferase; EC 2.4.1.226; UDP-GlcUA transferase)]
Species
Escherichia coli
Expression Region
1-686
Target Protein Sequence
MSILNQAINL YKNKNYRQAL SLFEKVAEIY DVSWVEANIK LCQTALNLSE EVDKLNRKAV IDIDAATKIM CSNAKAISLN EVEKNEIISK YREITAKKSE RAELKEVEPI PLDWPSDLTL PPLPESTNDY VWAGKRKELD DYPRKQLIID GLSIVIPTYN RAKILAITLA CLCNQKTIYD YEVIVADDGS KENIEEIVRE FESLLNIKYV RQKDYGYQLC AVRNLGLRAA KYNYVAILDC DMAPNPLWVQ SYMELLAVDD NVALIGPRKY IDTSKHTYLD FLSQKSLINE IPEIITNNQV AGKVEQNKSV DWRIEHFKNT DNLRLCNTPF RFFSGGNVAF AKKWLFRAGW FDEEFTHWGG EDNEFGYRLY REGCYFRSVE GAMAYHQEPP GKENETDRAA GKNITVQLLQ QKVPYFYRKK EKIESATLKR VPLVSIYIPA YNCSKYIVRC VESALNQTIT DLEVCICDDG STDDTLRILQ EHYANHPRVR FISQKNKGIG SASNTAVRLC RGFYIGQLDS DDFLEPDAVE LCLDEFRKDL SLACVYTTNR NIDREGNLIS NGYNWPIYSR EKLTSAMICH HFRMFTARAW NLTEGFNESI SNAVDYDMYL KLSEVGPFKH INKICYNRVL HGENTSIKKL DIQKENHFKV VNESLSRLGI KKYKYSPLTN LNECRKYTWE KIENDL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Glycosyltransferase that catalyzes elongation of chondroitin, a polysaccharide composed of a repeating disaccharide of N-acetylgalactosamine (GalNAc) and glucuronic acid (GlcUA) units, by alternatively transferring the GlcUA and GalNAc moiety from UDP-GlcUA and UDP-GalNAc to the non-reducing ends of the chondroitin chain. Each chondroitin unit has the composition beta-(1->4)-GlcUA-beta-(1->3)-GalNAc.
Protein Families
Glycosyltransferase 2 family, CS/HAS subfamily
Database Links

KEGG: ag:BAC00523

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