Recombinant Cyclin-dependent kinase inhibitor 1 (cki-1)

Code CSB-YP631501CXY
MSDS
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Source Yeast
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Code CSB-EP631501CXY
MSDS
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Source E.coli
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Code CSB-EP631501CXY-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP631501CXY
MSDS
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Source Baculovirus
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Code CSB-MP631501CXY
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
cki-1
Uniprot No.
Alternative Names
cki-1; T05A6.1Cyclin-dependent kinase inhibitor 1
Species
Caenorhabditis elegans
Expression Region
1-184
Target Protein Sequence
MSSARRCLFG RPTPEQRSRT RIWLEDAVKR MRQEESQKWG FDFELETPLP SSAGFVYEVI PENCVPEFYR TKVLTVRTTC SSLDISSTTL TPLSSPSTSD KEEPSLMDPN SSFEDEEEPK KWQFREPPTP RKTPTKRQQK MTDFMAVSRK KNSLSPNKLS PVNVIFTPKS RRPTIRTRSS CSPY
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Negative cell-cycle regulator that functions at the G1-to-S-phase transition. Required for suspension of the cell cycle in dauer larvae and starved L1 larvae. In vulval precursor cells (VPCs), a pathway of heterochronic genes acts via cki-1 to maintain VPCs in G1 during the L2 larval stage. Cul-2 may function in ubiquitin-mediated degradation by targeting cki-1 for degradation. Involved in distal tip cell development by repressing and modulating cye-1/cdk-2 activity levels in Z1.aa/Z4.pp and in Z1.ap/Z4.pa.
Gene References into Functions
  1. These data also suggest that integrin plays a major role in maintaining proper CKI-1/p27(KIP1) levels in the cell. PMID: 22879977
  2. BRAP-2 is necessary for preventing an inappropriate response to elevated levels of reactive oxygen species by countering premature activation of BRC-1 and CKI-1. PMID: 20207739
  3. CKI-1 cyclin-dependent kinase inhibitor has an essential embryonic role in cell cycle exit and morphogenesis. PMID: 12885569
  4. cki-1 is required to ensure the proper formation ans specification of the gonadal sheath cell precursors. PMID: 14597199
  5. Both cki-1 and cki-2 mediate cell cycle quiescence, but our genetic and phenotypic analyses demonstrate that they act within distinct pathways to exert control over the cell cycle machinery. PMID: 19597327

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Subcellular Location
Nucleus.
Protein Families
CDI family
Tissue Specificity
In embryos, expression is first seen in pharyngeal primordium and later in all differentiating cells. Post embryonic expression corresponds to developmental patterns of cell cycle progression in many tissues including sex myoblasts, distal tip cells, vulv
Database Links

KEGG: cel:CELE_T05A6.1

STRING: 6239.T05A6.1

UniGene: Cel.19541

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