Recombinant Danio rerio Achaete-scute homolog 1b (ascl1b)

Code CSB-YP835444DIL
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP835444DIL
MSDS
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Source E.coli
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Code CSB-EP835444DIL-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP835444DIL
MSDS
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Source Baculovirus
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Code CSB-MP835444DIL
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ascl1b
Uniprot No.
Alternative Names
ascl1b; ash; ashbAchaete-scute homolog 1b; Zash-1b
Species
Danio rerio (Zebrafish) (Brachydanio rerio)
Expression Region
1-195
Target Protein Sequence
MEATVVATTQ LTQDSFYQPF SESLEKQDRE CKVLKRQRSS SPELLRCKRR LTFNGLGYTI PQQQPMAVAR RNERERNRVK QVNMGFQTLR QHVPNGAANK KMSKVETLRS AVEYIRALQQ LLDEHDAVSA VLQCGVPSPS VSNAYSAGPE SPHSAYSSDE GSYEHLSSEE QELLDFTTWF DRYESGASMA TKDWC
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Transcriptional regulator. May mediate transcription activation by binding to the E box-containing promoter. Involved in neurogenesis. Involved in maintaining rhombomere boundaries in the hindbrain, probably via up-regulation of delta expression. May mediate transcription activation by binding to the E box-containing promoter.
Gene References into Functions
  1. Data demonstrate that MASH1/Ascl1a is a fundamental regulator of axonal growth across vertebrates and can induce modifications to the intrinsic state of neurons to promote functional regeneration in response to central nervous system injury. PMID: 25751153
  2. ascl1b/Ascl1 silencing by valproic acid results in the failure of serotonergic differentiation in a zebrafish model of fetal valproate syndrome PMID: 24135485
  3. The control of endocrine cell fate is instead fulfilled by two basic helix-loop-helix factors, Ascl1b and Neurod1 and NEUROG3 is not the unique pancreatic endocrine cell fate determinant in vertebrates. PMID: 23835295
Subcellular Location
Nucleus.
Tissue Specificity
In the 24 hours embryo, expressed in hindbrain close to the anterior and posterior boundaries of rhombomeres 2-6 and in ventral cells close to the floor plate of most rhombomeres. Also expressed in the telencephalon, diencephalon, tegmentum and spinal cor
Database Links

KEGG: dre:30478

STRING: 7955.ENSDARP00000022655

UniGene: Dr.318

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