Recombinant Danio rerio Homeobox protein OTX1 B (otx1b)

Code CSB-YP838638DIL
MSDS
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Source Yeast
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Code CSB-EP838638DIL
MSDS
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Source E.coli
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Code CSB-EP838638DIL-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP838638DIL
MSDS
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Source Baculovirus
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Code CSB-MP838638DIL
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
otx1b
Uniprot No.
Alternative Names
otx1b; otx1Homeobox protein OTX1 B; zOtx1; Orthodenticle homolog 1 B
Species
Danio rerio (Zebrafish) (Brachydanio rerio)
Expression Region
1-323
Target Protein Sequence
MMSYLKQPPY AMNGLGLSGA AMDLLHPSVG YPATPRKQRR ERTTFTRSQL DILEALFAKT RYPDIFMREE VALKINLPES RVQVWFKNRR AKCRQQQQSG SSTKTRPAKK KSSPTRESTG SESSGQFTPP AVSSAGSSSS SSSSTNNTGI SSTSTSISTV SSIWSPAISP GSAPPSVSLP EPVAPSNTSC MQRSVSGTAS STYPMPYNQT TGYSQGYPTP SGSYFSGVDC GSYLAPMHSH HHPHQLSPMT ASSMPTHPHH HISQSSGHHH HHHHQAYSGT GLAFNSSDCL DYKEQTASSW KLNFNTTDCL DYKDQASWRF QVL
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
May play a role in very early embryogenesis, gastrulation, and the development and subdivision of the diencephalon and the midbrain.
Gene References into Functions
  1. Foxa1 binds to the otx1b non-conserved enhancer to direct its activity in forebrain and otic vesicle of zebrafish at 24 hpf. PMID: 22011226
  2. otx1 expression can account for all major patterning differences between zebrafish and lampreys. PMID: 16510503
  3. Results suggest that for transcriptional activation of at least one gene in the cell aggregation pathway, zOtx1 need not bind directly to DNA, but does require helix 1 and 2 of its homeodomain to interact with an as yet undefined DNA binding protein. PMID: 19890851
Subcellular Location
Nucleus.
Protein Families
Paired homeobox family, Bicoid subfamily
Tissue Specificity
Embryonic expression is highly dynamic. In early gastrula embryos, expressed in the involuting presumptive anterior mesoderm. Found in the head rudiment at 9 hours of development, and at 12 hours of development expression increases at the caudal side but
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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