Recombinant Danio rerio Mast/stem cell growth factor receptor kita (kita), partial

Code CSB-YP810329DIL
MSDS
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Source Yeast
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Code CSB-EP810329DIL
MSDS
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Source E.coli
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Code CSB-EP810329DIL-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP810329DIL
MSDS
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Source Baculovirus
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Code CSB-MP810329DIL
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
kita
Uniprot No.
Alternative Names
kita; kit; sparse; Mast/stem cell growth factor receptor kita; SCFR; EC 2.7.10.1; Tyrosine-protein kinase Kit
Species
Danio rerio (Zebrafish) (Brachydanio rerio)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine kitlg/scf and plays a role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, and in mast cell development, migration and function. Required for the migration of cells in the melanocyte lineage and the survival of embryonic melanocytes. Required for the differentiation of some, but not all, melanocytes. Not essential for hematopoiesis or primordial germ cell development.
Gene References into Functions
  1. The expression of c-kit and beta-catenin suggests that erythropoietin may exert a role in regeneration reducing the extent of tubular damage from the outset after gentamicin administration. PMID: 27679401
  2. strong evidence for a Kit-mediated bi-directional communication system in the zebrafish ovarian follicle PMID: 23409152
  3. The role of ErbB and Kit signalling in establishing melanophore stem cells in zebrafish. PMID: 23364329
  4. The regeneration defect in kita mutants is not due to defects in melanocyte stem cells recruitment or in the proliferation, differentiation or survival of the daughter cells, but is instead due to a defect in stem cell establishment. PMID: 23364331
  5. Kita driven expression of oncogenic HRAS leads to early onset and highly penetrant melanoma in zebrafish PMID: 21170325
  6. melanocyte changes its response to Kit receptor signaling and function during development, first to promote migration, then to promote survival through distinct Kit-dependent mechanisms. PMID: 14512025
  7. kit is required for melanocyte differentiation in zebrafish when melanoblast development is delayed. PMID: 15300437
  8. Ectopic expression of foxd3 indicates that foxd3 promotes early melanophore death only when kit is inactive PMID: 19301400

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Subcellular Location
Cell membrane; Single-pass type I membrane protein.
Protein Families
Protein kinase superfamily, Tyr protein kinase family, CSF-1/PDGF receptor subfamily
Tissue Specificity
Expressed in cells of the neural crest-melanocyte lineage. In the embryo, also expressed in mesodermal cells that give rise to hematopoietic precursors, notochord, neural crest-derived cells of the branchial arches, pineal gland, retina and mechanorecepti
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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