Recombinant Danio rerio Probable fructose-2,6-bisphosphatase TIGAR B (tigarb)

Code CSB-YP772058DIL
MSDS
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Source Yeast
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Code CSB-EP772058DIL
MSDS
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Source E.coli
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Code CSB-EP772058DIL-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP772058DIL
MSDS
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Source Baculovirus
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Code CSB-MP772058DIL
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
tigarb
Uniprot No.
Alternative Names
tigarb; si:ch211-240j22.3; zgc:56074; Fructose-2,6-bisphosphatase TIGAR B; EC 3.1.3.46; TP53-induced glycolysis and apoptosis regulator B
Species
Danio rerio (Zebrafish) (Brachydanio rerio)
Expression Region
1-257
Target Protein Sequence
MLTFALTIVR HGETQYNRDK LLQGQGIDTP LSDTGHQQAA AAGRYLKDLH FTNVFVSNLQ RAIQTAEIIL GNNLHSSATE MILDPLLRER GFGVAEGRPK EHLKNMANAA GQSCRDYTPP GGETLEQVKT RFKMFLKSLF QRMLEEHGSA LSSAPSEADQ PVIAGLADDG AQNVPVHALM VSHGAFIRIS VRHLVEDLQC CLPAGLKMNQ VFSPCPNTGI SRFIFTIHRE ESVLRATRIQ GVFINRKDHL EEVKNSD
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Fructose-bisphosphatase hydrolyzing fructose-2,6-bisphosphate as well as fructose-1,6-bisphosphate. Acts as a negative regulator of glycolysis by lowering intracellular levels of fructose-2,6-bisphosphate in a p53/TP53-dependent manner, resulting in the pentose phosphate pathway (PPP) activation and NADPH production. Contributes to the generation of reduced glutathione to cause a decrease in intracellular reactive oxygen species (ROS) content, correlating with its ability to protect cells from oxidative or metabolic stress-induced cell death. May play a role in mitophagy inhibition.
Gene References into Functions
  1. Inactivation of TigarB gives rise to complete normalization of mitochondrial function, with resulting rescue of dopaminergic neurons in pink-/- larvae. PMID: 24027110
Subcellular Location
Cytoplasm. Nucleus. Mitochondrion.
Protein Families
Phosphoglycerate mutase family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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