Recombinant Danio rerio R-spondin-3 (rspo3)

Code CSB-YP728873DIL
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP728873DIL
MSDS
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Source E.coli
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Code CSB-EP728873DIL-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP728873DIL
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP728873DIL
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
rspo3
Uniprot No.
Alternative Names
rspo3; sb:cb387; zgc:162040; R-spondin-3; Cabriolet; Roof plate-specific spondin-3
Species
Danio rerio (Zebrafish) (Brachydanio rerio)
Expression Region
21-317
Target Protein Sequence
QHHGSRHRGN KQVSGVSSQG CQGGCQTCSV YNGCLTCKPK LFIHLERDGM RQIGVCLASC PNGFYGTRSP DRNDCIKCGS ECDSCFNRNF CLRCRAGSYL HKGKCMESCP DGLVPSDTKK ECVAACPALC DLCQNSDTCT RCVPGHFLHA GQCHHVCPDE FEPNDSMECI PTVHCEVSEW SEWGTCSRSG KTCGFKWGEE TRTRKVLQNP SPMGSPCPPT SEKRECFVKK KRCKPPKGQR RGEKKKRFNL QEKVTAEARR ERKREREKET IDREESENRN KTEQRRRRDQ SRDAGTV
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Activator of the canonical Wnt signaling pathway by acting as a ligand for lgr4-6 receptors, which acts as a key regulator of angiogenesis. Upon binding to lgr4-6 (lgr4, lgr5 or lgr6), lgr4-6 associate with phosphorylated lrp6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Acts both in the canonical. Wnt/beta-catenin-dependent pathway and in non-canonical Wnt signaling pathway. Acts as a key regulator of angiogenesis by controlling vascular stability and pruning: acts by activating the non-canonical Wnt signaling pathway in endothelial cells. Can also amplify Wnt signaling pathway independently of LGR4-6 receptors, possibly by acting as a direct antagonistic ligand to RNF43 and ZNRF3.
Gene References into Functions
  1. Rspo3 regulates dorsoventral and anteroposterior patterning in zebrafish embryos by negatively regulating the zygotic Wnt/beta-catenin signaling. PMID: 24918770
Subcellular Location
Secreted.
Protein Families
R-spondin family
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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