Recombinant Danio rerio Retinal homeobox protein Rx3 (rx3)

Code CSB-YP520357DIL
MSDS
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Source Yeast
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Code CSB-EP520357DIL
MSDS
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Source E.coli
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Code CSB-EP520357DIL-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP520357DIL
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP520357DIL
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
rx3
Uniprot No.
Alternative Names
rx3; Retinal homeobox protein Rx3
Species
Danio rerio (Zebrafish) (Brachydanio rerio)
Expression Region
1-292
Target Protein Sequence
MRLVGSQYKD MEDRLSPSAR LVRSPGSQTR IHSIESILGF KGETLFHPAF PYGSGKTGKD TEHLSPKKDS NKHFDGVCRS TVMVSPDLPD ADGGKLSDDE NPKKKHRRNR TTFTTFQLHE LERAFEKSHY PDVYSREELA LKVNLPEVRV QVWFQNRRAK WRRQEKLEVS SIKLQESSML SIPRSGPLSL GSGLPLEPWL TGPISTSSSP LQSLPSFITP QQAVPASYTP PQFLSSSTLN HSLPHIGAVC PPYQCSGFMD KFSLQEADPR NTSIASLRMK AKEHIQSIGK TW
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Plays a critical role in eye formation by regulating the initial specification of retinal cells and/or their subsequent proliferation.
Gene References into Functions
  1. Shh and Rx3 govern formation of a distinct progenitor domain that elaborates patterning through its anisotropic growth and differentiation. PMID: 27317806
  2. rx3 mutation leads to microphthalmia, incomplete eye maturation, and dramatic increases in apoptotic eye progenitors PMID: 15183718
  3. In addition to controlling optic lobe evagination and proliferation, chk/rx3 also determines cellular fate. PMID: 16300752
  4. The process segregating the telencephalic and eye fields is isolated from diencephalic patterning, and is mediated by Rx3. PMID: 16818451
  5. lmo4b has an essential role in forebrain development as a modulator of six3 and rx3 expression, and thus indirectly influences neural cell fate commitment, cell proliferation and tissue growth in the anterior CNS. PMID: 17692837

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Subcellular Location
Nucleus.
Protein Families
Paired homeobox family, Bicoid subfamily
Database Links

KEGG: dre:30474

STRING: 7955.ENSDARP00000069319

UniGene: Dr.540

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