Recombinant Danio rerio Rhombotin-2 (lmo2)

Code CSB-YP886095DIL
MSDS
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Source Yeast
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Code CSB-EP886095DIL
MSDS
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Source E.coli
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Code CSB-EP886095DIL-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP886095DIL
MSDS
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Source Baculovirus
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Code CSB-MP886095DIL
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
lmo2
Uniprot No.
Alternative Names
lmo2; si:dkey-10o6.5; Rhombotin-2; LIM domain only protein 2; LMO-2; zlmo2
Species
Danio rerio (Zebrafish) (Brachydanio rerio)
Expression Region
1-159
Target Protein Sequence
MASTIERKTL EANEEPVDEV LQMPPSLLTC GGCQQSIGDR FFLKAIEQYW HEDCLSCDLC GCRLGEVGRR LYYKLGRKLC RRDYLRLFGQ DGLCASCEKR IRAFEMTMRV RDKVYHLECF KCAACQKHFC VGDRYLLINS DIVCEQDIFE WTKLNGSIV
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcription factor that acts synergistically with tal1/scl in primitive and definitive hematopoiesis, and in endothelial development. Specifies mesodermal precursors to a hemangioblast cell fate. Hemangioblasts are bipotential precursors of blood and endothelium, and in the absence of hematopoietic induction cues such as gata1, tal1/scl-lmo2-induced haemangioblasts differentiate into endothelial cells.
Gene References into Functions
  1. The transcriptional factor LMO2 regulates endothelial proliferation and angiogenesis in vitro. Furthermore, LMO2 is required for angiogenesis and tissue healing in vivo. Thus, LMO2 is a critical determinant of vascular and tissue regeneration. PMID: 27792641
  2. a loss-of-function mutation in lmo2, a gene specifically required for hematopoiesis and vascular development, results in failure of optic fissure closure PMID: 22819672
  3. in the absence of inducers of erythroid or myeloid haematopoiesis, Scl/Tal1-Lmo2-induced haemangioblasts differentiate into endothelial cells PMID: 14602685
  4. Transcriptional regulation of lmo2 promoter during hematopoietic and vascular development in zebrafish is elucidated. PMID: 15893977
  5. Scl/Lmo2 complex does not appear to autoregulate, as neither gene's expression is affected by depletion of the other PMID: 17090656

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Subcellular Location
Nucleus.
Tissue Specificity
During embryogenesis, expressed in hematopoietic and endothelial precursors, including the two stripes of posterior and anterior lateral plate mesoderm at the 1-somite stage that give rise to the primitive blood.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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