Recombinant Danio rerio T-box transcription factor TBX20 (tbx20)

Code CSB-YP867431DIL
MSDS
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Source Yeast
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Code CSB-EP867431DIL
MSDS
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Source E.coli
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Code CSB-EP867431DIL-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP867431DIL
MSDS
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Source Baculovirus
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Code CSB-MP867431DIL
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
tbx20
Uniprot No.
Alternative Names
tbx20; T-box transcription factor TBX20; T-box protein 20; H15-related T-box transcription factor hrT
Species
Danio rerio (Zebrafish) (Brachydanio rerio)
Expression Region
1-446
Target Protein Sequence
MEYTSSPKPQ LSSRANAFSI AALMSSGKTK DKESEENTIK PLEQFVEKSS CHPNLGDLPP LETHSDFSSG GGTGSGAPLC TEPLIPTTPG VPSEEMAKIS CSLETKELWD KFHELGTEMI ITKSGRRMFP TIRVSFSGVD PDAKYIVLMD IVPVDNKRYR YAYHRSSWLV AGKADPPLPA RLYVHPDSPF TGEQLLKQMV SFEKVKLTNN ELDQHGHIIL NSMHKYQPRV HIIKKKDHTA SLLNLKSEEF RTFVFTETVF TAVTAYQNQL ITRLKIDSNP FAKGFRDSSR LTDIERESVE SLIHKHSYAR SPIRTYAGDE ETLGEEGHSA HSRGSAFTAS DNLSLSSWVT TTSGFSGFQH PQSLSAIGTS TASLASPLPH PIQGSLPPYS RLGMPLTPSA LASSMQATGP TFPSFHMPRY HHYFQQGPYA AIQGLRHSST VMTPFV
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Transcriptional regulator that may play a very early role in the differentiation of the cardiac precursors.
Gene References into Functions
  1. Tbx20 activity needs to be tightly fine-tuned to guarantee regular cardiomyocyte proliferation and embryonic heart growth in vivo. PMID: 27907103
  2. the identification of a zebrafish tbx20 mutation that results in an inactive, truncated protein lacking any functional domains, is reported. PMID: 27940156
  3. data demonstrate that elevated glucose alone induces cardiac defects in zebrafish embryos by altering the expression pattern of tbx5, tbx20, and has2 in the heart PMID: 20306498
  4. embryos lacking hrT function have dysmorphic hearts and an absence of blood circulation PMID: 12397116
Subcellular Location
Nucleus.
Database Links

KEGG: dre:57936

STRING: 7955.ENSDARP00000089658

UniGene: Dr.8231

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