Recombinant Dog Coagulation factor IX (F9)

Code CSB-YP007936DO
MSDS
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Source Yeast
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Code CSB-EP007936DO
MSDS
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Source E.coli
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Code CSB-EP007936DO-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP007936DO
MSDS
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Source Baculovirus
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Code CSB-MP007936DO
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
F9
Uniprot No.
Alternative Names
F9Coagulation factor IX; EC 3.4.21.22; Christmas factor) [Cleaved into: Coagulation factor IXa light chain; Coagulation factor IXa heavy chain]
Species
Canis lupus familiaris (Dog) (Canis familiaris)
Expression Region
40-452
Target Protein Sequence
Y NSGKLEEFVR GNLERECIEE KCSFEEAREV FENTEKTTEF WKQYVDGDQC ESNPCLNDGV CKDDINSYEC WCRAGFEGKN CELDVTCNIK NGRCKQFCKL GPDNKVVCSC TTGYQLAEDQ RSCEPAVPFP CGRVSVPHIS MTRTRAETLF SNMDYENSTE VEKILDNVTQ PLNDFTRVVG GKDAKPGQFP WQVLLNGKVD AFCGGSIINE KWVVTAAHCI EPDVKITIVA GEHNTEKREH TEQKRNVIRT ILHHSYNATI NKYNHDIALL ELDEPLTLNS YVTPICIADR EYSNIFLKFG SGYVSGWGRV FNKGRSASIL QYLKVPLVDR ATCLRSTKFT IYNNMFCAGF HEGGKDSCQG DSGGPHVTEV EGISFLTGII SWGEECAMKG KYGIYTKVSR YVNWIKEKTK LT
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Factor IX is a vitamin K-dependent plasma protein that participates in the intrinsic pathway of blood coagulation by converting factor X to its active form in the presence of Ca(2+) ions, phospholipids, and factor VIIIa.
Gene References into Functions
  1. Report relative sensitivity of whole blood clotting time and activated partial thromboplastin time for the detection of factor IX. PMID: 22482117
  2. Data indicate that the hyperactivating factor IX (FIX)-R338L mutation resulted in a robust 15-fold gain in potency and therefore provides a promising strategy to improve the efficacy, feasibility, and safety of hemophilia gene therapy. PMID: 23043073
  3. Data indicate that factor IX (FIX)-R338L provides an attractive strategy to safely enhance the efficacy of gene therapy for hemophilia B (HB). PMID: 22919027
  4. Describe proliferation of FIX-producing dog hepatocytes in mouse liver. PMID: 18449417
Involvement in disease
Defects in F9 are the cause of hemophilia B (HEMB).
Subcellular Location
Secreted.
Protein Families
Peptidase S1 family
Tissue Specificity
Detected in the liver.
Database Links
CUSABIO guaranteed quality
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