Recombinant Dog Inactive pancreatic lipase-related protein 1(PNLIPRP1)

In Stock
Code CSB-YP018263DO
Product Type Recombinant Protein
Size US$2959
Uniprot No. P06857
Relevance May function as inhibitor of dietary triglyceride digestion. Lacks detectable lipase activity towards triglycerides, diglycerides, phosphatidylcholine, galactolipids or cholesterol esters (in vitro).
Image
  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
  • Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP018263DO could indicate that this peptide derived from Yeast-expressed Canis lupus familiaris (Dog) (Canis familiaris) PNLIPRP1.
  • Based on the SEQUEST from database of Yeast host and target protein, the LC-MS/MS Analysis result of CSB-YP018263DO could indicate that this peptide derived from Yeast-expressed Canis lupus familiaris (Dog) (Canis familiaris) PNLIPRP1.
Storage Buffer Tris-based buffer,50% glycerol
Species Canis lupus familiaris (Dog) (Canis familiaris)
Purity Greater than 90% as determined by SDS-PAGE.
Sequence KEVCYEQIGCFSDAEPWAGTAIRPLKVLPWSPERIGTRFLLYTNKNPNNFQTLLPSDPSTIEASNFQTDKKTRFIIHGFIDKGEENWLLDMCKNMFKVEEVNCICVDWKKGSQTSYTQAANNVRVVGAQVAQMLSMLSANYSYSPSQVQLIGHSLGAHVAGEAGSRTPGLGRITGLDPVEASFQGTPEEVRLDPTDADFVDVIHTDAAPLIPFLGFGTSQQMGHLDFFPNGGEEMPGCKKNALSQIVDLDGIWEGTRDFVACNHLRSYKYYSESILNPDGFASYPCASYRAFESNKCFPCPDQGCPQMGHYADKFAVKTSDETQKYFLNTGDSSNFARWRYGVSITLSGKRATGQAKVALFGSKGNTHQFNIFKGILKPGSTHSNEFDAKLDVGTIEKVKFLWNNNVVNPTFPKVGAAKITVQKGEEKTVHSFCSESTVREDVLLTLTPC
Research Area Signal Transduction
Source Yeast
Gene Names PNLIPRP1
Protein Names Short name:PL-RP1
Expression Region 18-467aa
Tag Info N-terminal 6xHis-tagged
Mol. Weight 51.7 kDa
Protein Description Full Length of Mature Protein
Storage The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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Function May function as inhibitor of dietary triglyceride digestion. Lacks detectable lipase activity towards triglycerides, diglycerides, phosphatidylcholine, galactolipids or cholesterol esters (in vitro).
Subcellular Location Secreted
Protein Families AB hydrolase superfamily, Lipase family
Tissue Specificity Detected in pancreas (at protein level).
Database Links

KEGG: cfa:404010

STRING: 9615.ENSCAFP00000017453

UniGene: Cfa.3881

Q&A and Customer Reviews

Q&A
Q:

I bought cPL recombinant protein Recombinant Dog Inactive pancreatic lipase-related protein 1(PNLIPRP1) N-terminal 6xHis-tagged (03613) CSB-YP018263DO (order M1-69098)
I tried to test it and diluted the protein 500ug by 1ml water but the powder is not diluted absolutely.
I did vortexing to protein and waited for a while.
The pallet is not dissolved until now. Could you tell me the reason?

A:
Thanks for your inquiry. Our tech team confirms that we test the reconstitution before shipping. This lyophilized protein could be dissolved in deionized sterile water well.
The isoelectric point(pI) of this protein is 6.1, it’s a acidic protein. Our tech team suggest you to add 0.1M NaHCO3 to help resuspension. Dissolving the protein into sterile water, and then adding 0.1M NaHCO3 drop by drop. Stir gently and dissolve at room temperature 30 min. If it still does not work, pls send the picture of the protein after dissolution for us to analyze.
Here is information about reconstitution for your reference.
Reconstitution
The reconstitution method is the same for all proteins. Please use deionized sterile water to reconstitute proteins. (Dissolve completely. The concentration could depend on your experiment demand.)
It is better that concentration does not be lower than 0.1mg/ml. Proteins will be unstable if they're too diluted. Generally, we recommend 0.1-1.0mg/ml concentration. If you have special requirements for concentration, then you could dilute the protein according to your experiment demand.
We haven’t studied whether the optimal concentration range will vary among different hosts such as e. coli, yeast, mammalian or baculovirus, so we can’t provide usable advice.
If you will use immediately after dissolving one protein, then you don't need to add glycerol. If you won't use the protein in short term, we suggest to add some glycerol for storage. We suggest you to reconstitute suitable amount of protein and store the remaining protein in lyophilized powder. You could remark "The lyophilized powder should be packed into multiple vials" when placing order. The amount of lyophilized powder in each vial will depend on your experiment demand.
In principle, the final concentration of glycerol from 5% to 50% all will be OK. Our default final concentration of glycerol is 50%. You could use it as reference.
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