Recombinant Dog Inactive pancreatic lipase-related protein 1 (PNLIPRP1)

Thanks for your inquiry. Our tech team confirms that we test the reconstitution before shipping. This lyophilized protein could be dissolved in deionized sterile water well.
The isoelectric point(pI) of this protein is 6.1, it’s a acidic protein. Our tech team suggest you to add 0.1M NaHCO3 to help resuspension. Dissolving the protein into sterile water, and then adding 0.1M NaHCO3 drop by drop. Stir gently and dissolve at room temperature 30 min. If it still does not work, pls send the picture of the protein after dissolution for us to analyze.
Here is information about reconstitution for your reference.
Reconstitution
The reconstitution method is the same for all proteins. Please use deionized sterile water to reconstitute proteins. (Dissolve completely. The concentration could depend on your experiment demand.)
It is better that concentration does not be lower than 0.1mg/ml. Proteins will be unstable if they're too diluted. Generally, we recommend 0.1-1.0mg/ml concentration. If you have special requirements for concentration, then you could dilute the protein according to your experiment demand.
We haven’t studied whether the optimal concentration range will vary among different hosts such as e. coli, yeast, mammalian or baculovirus, so we can’t provide usable advice.
If you will use immediately after dissolving one protein, then you don't need to add glycerol. If you won't use the protein in short term, we suggest to add some glycerol for storage. We suggest you to reconstitute suitable amount of protein and store the remaining protein in lyophilized powder. You could remark "The lyophilized powder should be packed into multiple vials" when placing order. The amount of lyophilized powder in each vial will depend on your experiment demand.
In principle, the final concentration of glycerol from 5% to 50% all will be OK. Our default final concentration of glycerol is 50%. You could use it as reference.