Product Details

Purity

Greater than 90% as determined by SDS-PAGE.

Target Names

PNLIPRP1

Uniprot No.

P06857

Research Area

Signal Transduction

Alternative Names

PNLIPRP1; PLRP1; Inactive pancreatic lipase-related protein 1; PL-RP1

Species

Canis lupus familiaris (Dog) (Canis familiaris)

Source

E.coli

Expression Region

18-467aa

Target Protein Sequence

KEVCYEQIGCFSDAEPWAGTAIRPLKVLPWSPERIGTRFLLYTNKNPNNFQTLLPSDPSTIEASNFQTDKKTRFIIHGFIDKGEENWLLDMCKNMFKVEEVNCICVDWKKGSQTSYTQAANNVRVVGAQVAQMLSMLSANYSYSPSQVQLIGHSLGAHVAGEAGSRTPGLGRITGLDPVEASFQGTPEEVRLDPTDADFVDVIHTDAAPLIPFLGFGTSQQMGHLDFFPNGGEEMPGCKKNALSQIVDLDGIWEGTRDFVACNHLRSYKYYSESILNPDGFASYPCASYRAFESNKCFPCPDQGCPQMGHYADKFAVKTSDETQKYFLNTGDSSNFARWRYGVSITLSGKRATGQAKVALFGSKGNTHQFNIFKGILKPGSTHSNEFDAKLDVGTIEKVKFLWNNNVVNPTFPKVGAAKITVQKGEEKTVHSFCSESTVREDVLLTLTPC
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

65.7kDa

Protein Length

Full Length of Mature Protein

Tag Info

N-terminal 6xHis-SUMO-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

The process of expressing this Dog PNLIPRP1 recombinant protein in E.coli expression system requires the following steps: use of competent E. coli cells to take up DNA sequence of interest, the exogenous plasmid is transferred into the cell, and the transcription and translation are directly replicated in the cell, selection of transformed E. coli using a selection marker, expansion of selected E. coli to a higher scale in appropriate culture media, such as classic LB options Growth Systems and lastly isolation and purification.And we finally get a PNLIPRP1 protein with N-terminal 6xHis-SUMO tag and it is determined by SDS-PAGE with a purity of 90%+.

PNLIPRP1 (PLRP1) is a protein coding gene that encodes Inactive pancreatic lipase-related protein 1. According to some studies, PNLIPRP1 may have the following features.
The loss of PNLIPRP1 and NR1I3 was mainly associated with specific eating habits. The exons of these diabetic patients showed the rs2305205 mutation in the PNLIPRP1 gene and the rs778701848 mutation in the CAMKK2 gene. PNLIPRP1 showed no lipase activity against all known substrates. PNLIPRP1 acts as a negative regulator of pancreatic lipase activity by competing with PL to occupy colonic lipase.

Target Background

Function

May function as inhibitor of dietary triglyceride digestion. Lacks detectable lipase activity towards triglycerides, diglycerides, phosphatidylcholine, galactolipids or cholesterol esters (in vitro).

Subcellular Location

Secreted.

Protein Families

AB hydrolase superfamily, Lipase family

Tissue Specificity

Detected in pancreas (at protein level).

Database Links

KEGG: cfa:404010

STRING: 9615.ENSCAFP00000017453

UniGene: Cfa.3881

Code	CSB-EP018263DO
Abbreviation	Recombinant Dog PNLIPRP1 protein
MSDS	MSDS Datasheet
Size	US$388
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Recombinant Dog Inactive pancreatic lipase-related protein 1 (PNLIPRP1)

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