Recombinant Drosophila melanogaster 14-3-3 protein epsilon (14-3-3epsilon)

Code CSB-YP026287DLU
MSDS
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Source Yeast
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Code CSB-EP026287DLU
MSDS
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Source E.coli
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Code CSB-EP026287DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP026287DLU
MSDS
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Source Baculovirus
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Code CSB-MP026287DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
14-3-3epsilon
Uniprot No.
Alternative Names
14-3-3epsilon; 14-3-3e; SR3-9; CG3119614-3-3 protein epsilon; Suppressor of Ras1 3-9
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-262
Target Protein Sequence
MTERENNVYK AKLAEQAERY DEMVEAMKKV ASMDVELTVE ERNLLSVAYK NVIGARRASW RIITSIEQKE ENKGAEEKLE MIKTYRGQVE KELRDICSDI LNVLEKHLIP CATSGESKVF YYKMKGDYHR YLAEFATGSD RKDAAENSLI AYKAASDIAM NDLPPTHPIR LGLALNFSVF YYEILNSPDR ACRLAKAAFD DAIAELDTLS EESYKDSTLI MQLLRDNLTL WTSDMQAEEV DPNAGDGEPK EQIQDVEDQD VS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Positively regulates Ras-mediated pathways. Acts downstream or parallel to Raf, but upstream of nuclear factors in Ras signaling. Three mutants have been isolated, that suppress the rough eye phenotype caused by mutated Ras1 (sev-Ras1 v12). Inhibits yki activity by restricting its nuclear localization. Together with pav, has a role in the inhibition of microtubule sliding during neurite outgrowth.
Gene References into Functions
  1. 14-3-3epsilon has a critical role in cellular metabolism involving either molecular crosstalk with the EcR or direct interaction with metabolic proteins PMID: 28365999
  2. role of 14-3-3epsilon in germ cell migration PMID: 22666326
  3. 14-3-3epsilon acts as a biochemical control point for axon guidance in Drosophila, silencing Plexin A repulsive axon guidance and regulating a semaphorin repulsion/integrin adhesion switch. PMID: 22500634
  4. We conclude that 14-3-3epsilon is required for Rab11-positive vesicle function, which in turn enables antimicrobial peptide secretion during an innate immune response. PMID: 21670199
  5. Drosophila 14-3-3/PAR-5 is an essential mediator of PAR-1 function in axis formation. PMID: 12431373
  6. Serves as a central modulator of forkhead transcription factor FoxO activity in the regulation of growth, cell death and longevity in vivo. PMID: 18665908

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Protein Families
14-3-3 family
Database Links

KEGG: dme:Dmel_CG31196

STRING: 7227.FBpp0082987

UniGene: Dm.2357

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